红花石蒜（Lycoris radiata）“花叶期不遇”现象机制的研究

红花石蒜（Lycoris radiata）为中国特色球根花卉，观赏价值较高。研究其“花叶期不遇”现象的调控机制，对有效调控花期及其产业化开发具有重要意义。根据前期多年的物候观察与栽培研究推测，温度很可能是主导红花石蒜“花叶期不遇”习性的环境因子。本项目拟首先通过对红花石蒜的物候与小气候监测、花发育形态解剖、花发育生理等研究，掌握红花石蒜“花叶期不遇”习性与温度的相关性；然后，分别采用6种不同温度的盆栽和8种贮藏处理，研究红花石蒜其最适的生长、胁迫及休眠诱导的温度范围及其“花叶期不遇”现象的有效调控途径；再结合红花石蒜花发育生理、生化指标的动态监测及不同处理下差异蛋白质组学分析，探寻其“花叶期不遇”的温度响应机制。

Lycoris radiata is Chinese characteristic bulbous flowers with high ornamental value. It would be valuable to study its growth habit of florescence without leaf and the control mechanirm for effective regulation of flowering and its industrialization development.. According to the previous studies about the phenology observation and cultivation. Project team speculated that the temperature is likely to be the key factor to decided the growth rhythm of L.radiata.To fully understand the relation between the growth rhythm of L.radiata and the temperature and its physiological mechanism. The project will conduct a series of experiments, which sequentially was the phenology and microclimate monitoring, the morphology anatomy, physiology and biochemistry of flower development, and others.. Then, the cultivation experiments under 6 different temperature control and 8 different temperature storage would be conducted aimed to exploring its optimum growth, stress and dormancy induction temperature range, and effective regulatory pathways.. Finally, We study hoped to exploring the internal cause of growth rhythm and the physiological response mechanism of L.radiata to the temperature by combing the flower bud differentiation observation, the bulb physiological and biochemical substances determination and the analysis of differential proteomics under different treatments.