环状RNA(circular RNA)与动脉粥样硬化(AS)性心脑血管病密切相关，但circRNA在AS发病中的机制仍待详细研究。我们发现circRNA0141620在颈动脉AS斑块中高表达，miR196a-5p低表达，下游分子TLR5高表达，脂质转运蛋白ABCA1、ABCG1低表达；据此，我们推断circRNA可能通过ceRNA调控网络作用于下游TLR5炎症信号通路，促进血管巨噬细胞炎症反应和脂质沉积，发挥促AS形成的作用。本课题采用人AS斑块及正常动脉组织，明确circRNA信号通路分子的具体变化。通过细胞实验明确circRNA信号通路细胞水平的分子机制；采用基因编辑敲除circRNA、miR196a、TLR5，明确circRNA-miR196a-5p-TLR5信号通路在AS形成中的作用。本实验通过揭示circRNA对AS形成的促进作用及机制，为颈动脉粥样硬化防治提供新的靶点。

Circular RNA is closely related to atherosclerosis (AS) cardio-cerebral vascular disease, but the mechanism of circRNA in the pathogenesis of AS remains to be studied in detail. We found that circRNA0141620 is highly expressed in carotid AS plaques, low expression of miR196a-5p, high expression of TLR5 in downstream molecules, and low expression of lipid transporters ABCA1 and ABCG1. Based on this, we conclude that circRNA may act on downstream TLR5 inflammatory signaling pathway through ceRNA regulatory network, promoting Vascular macrophage inflammatory response and lipid deposition play a role in promoting AS formation. This topic uses human AS plaques and normal arterial tissue to identify specific changes in the circRNA-141620 signaling pathway molecule. The molecular mechanism of the circRNA signaling pathway at the cellular level was confirmed by cell experiments; gene editing was used to knock out circRNA, miR196a, and TLR5, and the role of the circRNA-miR196a-5p-TLR5 signaling pathway in AS formation was clarified. This experiment provides a new target for the prevention and treatment of carotid atherosclerosis by revealing the promoting effect and mechanism of circRNA on AS formation.