针对肺炎克雷伯菌的多重感染及免疫逃逸机制，研发新型疫苗对有效控制日趋严峻的K.pneumoniae耐药性发展和爆发流行具有重要意义。课题组筛选构建新型K.pneumoniae疫苗，其免疫后攻毒保护率大于80%，但其免疫保护机制并不清楚。课题组前期研究结果提示天然免疫记忆及CD4+T细胞免疫应答可能在该疫苗的免疫保护中发挥了重要作用。为进一步阐明该保护机制，拟通过转录水平(转录芯片)、蛋白水平(细胞因子芯片)、细胞水平(条件性阻断、交叉过继转移模型)、整体水平(基因敲除模型)，全面系统地分析天然免疫记忆(单核/巨噬细胞、中性粒细胞、NK细胞)及Th细胞免疫(Th1、Th9、Th17和Th22)应答在K.pneumoniae疫苗中的免疫保护作用及机制，为合理设计更加安全、有效的K.pneumoniae疫苗提供重要的实验基础及理论依据。

In view of multiple infections and immune escape mechanisms of Klebsiella pneumoniae, the development of new vaccines is effective in controlling the increasingly severe K. Pneumoniae resistance development and outbreaks are of great importance. The research group screened and constructed a novel K. Pneumoniae vaccine, the post-immunization challenge protection rate is greater than 80%, but the mechanism of immune protection is not clear. Our previous results suggest that innate immune memory and CD4 + T cell immune response may play an important role in the immune protection of the vaccine. To further elucidate this protective effect, it is proposed to comprehensively and systematically analyze innate immune memory (monocytes/macrophages, neutrophils, NK cells) and acquired cellular immune (Th1, Th9, Th17, and Th22) responses at the transcriptional level (transcription microarray), protein level (cytokine microarray), cellular level (blocking, cross-adoptive transfer model), and global level (knockout model). The immunoprotective effect and mechanism of pneumoniae vaccine for the rational design of safer and more effective K. Pneumoniae vaccine provides an important experimental basis and theoretical basis.