耐甲氧西林金黄色葡萄球菌(MRSA)是引起医院和社区获得性感染疾病的主要致病菌。发展基于多价保护性的亚单位融合疫苗对于控制MRSA的感染与爆发性流行具有重要的意义。纤连蛋白结合蛋白A（FnBPA) 在金黄色葡萄球菌的致病中具有重要作用,课题组前期发现，FnBPA具有良好的免疫原性，对MRSA感染具有较强的免疫保护效果。然而，FnBPA的N端基因序列的多态性和该重组表达的蛋白分子量较大，使其成为多价亚单位融合疫苗的组分具有一定的困难，筛选保守的免疫显性片段是解决这一问题的重要途径。在此基础上，将进一步阐明FnBPA的免疫显性片段的保护性适应免疫应答谱学特征及作用机制。通过全面分析体液免疫及细胞免疫（Th1和Th17）应答在MRSA疫苗中的免疫保护作用及其应答机制，为深化对对MRSA感染免疫保护机制的认识，合理设计更加安全、有效的MRSA基因工程疫苗提供重要的实验基础。

Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional ‘micro¬bial surface components recognizing adhesive matrix molecules’ (MSCRAMMs). It is one of the most important adhesin molecules involved in the initial adhesion steps of S. aureus infection. However, FnBPA is a high-molecular-weight protein and Sequence diversity in the N term limit its vaccine application in MRSA infection diseases control. Mapping the conserved immunodominant regions of FnBPA is important for developing polyvalent subunit fusion vaccines against MRSA infections. This information will be important for further developing anti- S. aureus polyvalent subunit fusion vaccines. To further investigate the mechanism of protective immunity response, we will overally and systematically analyze immunoprotective effect and response mechanism from humor immunity and celluar immunity in the MRSA vaccine. It is important for the further understand MRSA infection pathogenic and acknowlege immunoprotection mechanism, affording experiment foundation for design more safety and effective MRSA genetic engineering multivalent subunit vaccine.