开发新型水凝胶是生物医学领域的重要课题。目前用于细胞三维培养的合成水凝胶大多基于传统的单体聚合方法来制备，构筑与细胞外基质类似的合成胶体水凝胶一直是领域难点。本项目拟建立由胶体基元构建新型动态胶体水凝胶的策略；利用其动态性质开发对胶体水凝胶进行微加工的技术和探索胶体水凝胶的仿生细胞外基质应用。主要包括以下几个方面内容：(1)优化设计合成胶体构筑基元，选择高效的胶体基元表面修饰方法，实现胶体构筑基元的高质量、低成本、高产量制备；(2)调控超分子作用力，结合外场等因素，精细调控胶体构筑基元组装过程，实现动态、拓扑形貌可调的胶体水凝胶的可控制备，探究胶体水凝胶特有的构效关系；(3)协同胶体水凝胶的动态性质和微加工技术，实现胶体水凝胶的结构和性能在时间和空间上的调节；(4)利用其仿生细胞外基质特性探索胶体水凝胶在干细胞和肿瘤细胞的三维无酶培养及仿生微器官模型构筑等方面的应用。

Development of new hydrogels is the central project of importance in the biomedical field. The synthetic hydrogels for the three-dimensional (3D) cell culture are mostly prepared from traditional molecular monomers, so there are challenges on the preparation of extracellular-matrix (ECM)-like synthetic hydrogel from colloidal building blocks. In this proposal, we plan to synthesize new types of dynamic hydrogels assembled from colloidal building blocks. By using their dynamic inherence, the microfabrication techniques will be investigated to fabricate complex microarchitectures from the colloidal hydrogels, and the potential of the colloidal hydrogels as biomimetic ECM will be explored. This proposal mainly includes four parts. We will firstly design and synthesize the colloidal building blocks and modify the colloidal building blocks by functional polymers, obtaining the high-quality colloidal building blocks in a high throughput and low-cost fashion. Second, the colloidal hydrogels with dynamic and controllable architectures will be prepared by finely tailoring the driven forces in the process of self-assembly of colloidal building blocks, moreover, the relationship between structures and properties of the colloidal hydrogels will be explored. Third, using synergy of their dynamic properties and microfabrication techniques, the microarchitectures from the colloidal hydrogels will be fabricated to tune the structures and properties of the colloidal hydrogels in the spatial and temporal space. Finally, as a biomimetic ECM, the colloidal hydrogels will be used in the field of 3D enzyme-free cell culture of stem cells and cancer cells， and fabrication of biomimetic micro-organ models.