Ｄ-阿拉伯糖醇是一种重要的功能性五碳多元糖醇，在食品、医药、化工等领域具有广泛应用。以葡萄糖为底物，利用高渗酵母发酵生产 D-阿拉伯糖醇是近年来的热点研究领域之一，但是其合成机制以及如何实现高效发酵合成等关键科学问题尚未得到明确而合理的解答。在课题组前期相关研究积累的基础上，本项目以鲁氏酵母RJM-107为研究对象，通过转录组学和蛋白质组学分析技术，确定与D-阿拉伯糖醇合成相关的关键基因和关键蛋白；其次，利用13C标记的代谢流技术，揭示D-阿拉伯糖醇合成的代谢通路、关键代谢节点和限速步骤；随后，通过转录组-蛋白组-代谢流的比较关联分析，解析鲁氏酵母利用葡萄糖发酵合成D-阿拉伯糖醇的分子机制；最后，采用CRISPR-Cas等技术强化葡萄糖到D-阿拉伯糖醇通路的代谢流量，完成鲁氏酵母的代谢工程改造，实现以葡萄糖为底物的D-阿拉伯糖醇的高效发酵合成。从而为D-阿拉伯糖醇的工业化发酵生产提供参考。

D-arabitol is an important functional five-carbon polyhydric sugar alcohol, which is widely used in food, medicine, chemical and other fields. The production of D-arabitol by osmophilic yeast using glucose as substrate is one of the hot research fields in recent years. However, the key scientific issues such as its synthesis mechanism and how to achieve efficient biosynthesis by fermentation have not been answered clearly and reasonably. Based on the accumulation of many related researches in the previous period, this project uses Zygosaccharomyces rouxii RJM-107 as the starting stain. The key genes and proteins affecting D-arabitol synthesis are identified by means of transcriptomics and proteomic techniques. Subsequently, the metabolic pathway, key metabolic node and rate-limiting step of D-arabitol biosynthesis are determined by the method of metabolic flow using 13C-labeled glucose. Thereafter, the molecular mechanism of the D-arabitol synthesis by fermentation from glucose is formulated comprehensively through the comparison analysis of transcriptome-proteome-metabolic flux. Finally, the metabolic flux of glucose to the D-arabitol pathway is enhanced by using CRISPR-Cas and other techniques. And the metabolic engineering modification of RJM-107 strain was completed to achieve the high-efficiency fermentation of D-arabitol from glucose. The results of this project provide a theoretical and practical reference for the industrial production of D-arabitol by fermentation.