近年来国内外研究表明神经干细胞移植对脑出血后的神经保护和功能改善具有显著作用，但目前仍然存在移植干细胞存活率低、增殖分化少等诸多问题有待解决。我们在前期curcumin神经保护机制研究的基础上，为进一步探索curcumin运用于脑出血后神经干细胞移植治疗的可行性及其作用机制，将探讨并验证：（1）curcumin通过抑制铁离子诱导的神经干细胞凋亡发挥保护作用，促进细胞存活、增殖、分化和迁移；curcumin预处理可更显著改善脑出血后神经干细胞移植的预后；（2）采用液相质谱串联质谱筛选curcumin干预后与干细胞存活、增殖及分化相关且表达显著变化的候选蛋白，结合筛选和验证信号通路的变化；采用慢病毒转染调控白表达、特定信号通路的抑制剂/激动剂等方法确定候选蛋白的功能及curcumin作用的靶点。本课题的开展将有望阐明curcumin作用的分子机制，促进其运用于脑出血后神经干细胞移植的转化研究。

In recent years, domestic and overseas studies indicated that neural stem cell transplantation after intracerebral hemorrhage shows significant neruoprotection and neurological improvement. However, the low rate of survival, proliferation and differentiation in transplanted stem cells remained existing. On the basis of the neuroprotective research of curcumin, aiming to explore the role and mechanism of curcumin in neural stem cell transplantation after intracerebral hemorrhage, we will discuss and confirm that: (1) Curcumin shows a protective effect by inhibiting iron-induced apoptosis of neural stem cells; Curcumin promotes neural stem cell proliferation, differentiation and migration; Curcumin-pretreated neural stem cells may improve neurological function after intracerebral hemorrhage and further improve the overall outcome; (2) By using LC-MS/MS, the candidate protein related with survival/proliferation/differentiation in curcumin-treated neural stem cell will be identified; Combined with screening in cell level , the signal pathway of curcumin will be studied; By using lentivirus transfection to regulate the candidate gene expression, or by using specific inhibitors and activators, the targets and pharmacological mechanism of curcumin will be studied. This topic is expected to clarify the molecular mechanisms of pharmacological action of curcumin, and to guide the translational research of curcumin treatment in transplantation therapy of neural stem cells after intracerebral hemorrhage.