基于“基因表达-靶向组分-肠道菌群” 关联性分析探讨复方杏香兔耳风对宫颈炎微环境的影响

宫颈炎位列十大妇科病之首，严重危害妇女健康。由于细菌的耐药性和抗生素的副作用，单靠化学药治疗已无法解决宫颈炎的治疗难题。当前，中药及其复方在抗感染疾病治疗中发挥了积极作用，其中复方杏香兔耳风治疗宫颈炎疗效确切，但物质基础和机制尚不明确。体内微生态系统对宫颈炎等炎症疾病发生、发展和治愈都具有重大影响。在前期研究中，我们发现复方杏香兔耳风能引起宫颈炎微环境miR-1976等mRNA表达改变，复方杏香兔耳风很可能是通过靶向组分对宫颈和肠道微环境中的“基因表达及肠道菌群”产生影响而发挥作用。本项目拟在前期对杏香兔耳风有效成分、体内代谢、宫颈和卵巢组织分布、抗菌机制和耐药特征研究的基础上，进一步从微观角度的“基因表达-靶向组分-肠道菌群”关联性分析探讨复方杏香兔耳风对宫颈炎微环境的影响，为今后临床使用复方杏香兔耳风制剂及其二次开发和进一步探索中药及其复方抗宫颈炎等感染性疾病的科学内涵奠定基础。

Cervicitis is one of the top 10 gynecologic disease which seriously affected womens health. Because of resistance of bacteria and the side effects of antibiotic，the difficult problem for cervicitis clinical treatment can't be solved by western medicine. Traditional Chinese medicine (TCM) and its compound played a positive role in the treatment infectious diseases at present. Among these (TCM) and its compound, Fufang Xingxiangtuerfeng（FFXXTEF） has displayed positive effects，but the material basis and mechanism is not clear. In vivo microenvironment play an important role in the development and healing on cervicitis and other inflammatory diseases. In the former work of our laboratory, we had discovered and identified that FFXXTEF can arouse the change of expression miR-1976 mRNA in cervicitis microenvironment. The good therapeutic effect of FFXXTEF could be it’s effection of on the correlation of "Gene Expression-Target Component-Intestinal Flora. On the basis of previous work in active ingredients, metabolic,cervical and ovarian tissue distribution, antibacterial mechanism and drug resistance characteristics research, we will study the the effects of FFXXTEF on microenvironment of crvicitis by using the correlation of "Gene Expression-Target Component-Intestinal Flora". This study will be beneficial to the future clinical use of FFXXTEF and its secondary development, to further understanding of the scientific connotation of Traditional Chinese medicine (TCM) resistance to infectious diseases.