针刺联合壮医药线点灸法治疗失眠的机制研究

失眠严重影响人们的身心健康，不良刺激导致应激反应性增强使失眠的患病率上升。近年研究表明增食素（orexin）参与睡眠-觉醒周期、应激反应的调节，我们在前期对失眠的研究观察到针刺联合壮医药线点灸能下调orexin mRNA表达，抑制下丘脑一垂体一肾上腺轴（HPA axis）激活，降低应激反应性，推测其机制可能是通过调控orexin及其调节的应激信号，进而调节HPA axis 及蓝斑-交感神经轴（LC-NE axis）的应激反应性。本课题在此基础上，应用针刺"神门"、"百会"联合壮医药线点灸"足三里"干预失眠模型大鼠，采用定量real-time PCR、ELISA 法、EEG、EMG技术检测orexin及其调控的HPA axis和LC-NE axis信号通路分子的变化，研究其治疗失眠的作用机制。研究结果为针刺"神门"、"百会"联合壮医药线点灸"足三里"治疗失眠的临床应用提供理论指导。

Insomnia impairs seriously people's health in many ways, and pessimal stimulations often cause the prevalence of insomnia rising due to intense stress reaction. Recent researches have indicated that orexin was playing an important pole on adjusting sleep-wakefulness period and stress reaction . In our early researches we noticed that acupuncture combined with medicated thread moxibustion of Zhuang nationality medicine could down-regulate expression of orexin mRNA, inhibit activation of HPA axis, lessen stress reaction and so that can cure insomnia, so we had a hypothesis about its therapeutic mechanism where it can adjust orexin and other related signal moles , and further regulate stress reaction of HPA axis and LC-NE axis. Based on early research results ,this programme continued exploring and discussing the therapeutic mechanism by applying acupuncturing "Shenmen" point ,"Baihui"point combined with "Zusanli" point medicated thread moxibustion of Zhuang nationality medicine treating rats with insomnia , applying quantitative real-time PCR, ELISA, EEG,EMG techniques or methods to detect changes of orexin and signal moles of stress reaction of HPA axis and LC-NE axis. The results of the programme will provide basic theoretic guide for its treatment of insomnia.