急性肺损伤（ALI）后的间质纤维化是ALI加重与死亡的重要原因，上皮间质转化（EMT）是间质纤维化的重要机制，上皮剪切调节蛋白（ESRP）则是EMT发生的关键因子，但ESRP是否参与了ALI的间质纤维化尚未见文献报道。本项目拟采用LPS致急性肺损伤、Rosa 26与SPC-Cre转基因小鼠等模型，应用病理学、免疫组织化学、RT-PCR、荧光定量PCR、Western Blot等技术，明确急性肺损伤过程中EMT的发生发展规律，以及ESRP在其中的作用与地位，并探讨ESRP表达变化的调控机制，从而为重度急性肺损伤的救治提供新的可能途径。

The interstitial fibrosis after acute lung injury(ALI) is an important cause of ALI exacerbation and death. The epithelial mesenchymal transition (EMT) is a crucial mechanism of interstitial fibrosis, and the epithelial splicing regulatory protein (ESRP) is a key factor in the occurrence of EMT. However, whether ESRP is involved in the interstitial fibrosis of ALI is still unknown. In this study, LPS will be used to induce acute lung injury in Rosa 26 and SPC-Cre transgenic mice models. Moreover, we will apply the techniques of pathology, immunohistochemistry, RT-PCR, fluorescence quantitative PCR, western blot and so on, to clarify the occurrence and development of EMT in the process of acute lung injury, as well as the role of ESRP during this process, and to explore the molecular mechanism in regulation of ESRP expression. Our resules will provide a new method for the treatment of severe acute lung injury.