本项目的主要研究内容是喀斯特洞穴内水体重金属分布特征研究及其人体健康风险评价；喀斯特洞穴内食用水生动物体内重金属的分布特征、富集研究及其健康风险评价；喀斯特洞穴水生动物指示种研究；喀斯特洞穴水体中铅同位素含量及利用Pb同位素指纹特征对洞穴邻近区域的汽车尾气排放、燃煤释放、工业生产排放、农药排放等进行示踪，揭示污染物的来源及部分研究对象的相对贡献率。通过本项目研究，基本了解研究区域内喀斯特洞穴水生动物的种类（或类群）组成及相对数量；了解洞穴水生动物和洞穴水体的重金属污染现状及对人体的健康危害风险；查明洞穴水环境的重金属污染源，确定洞穴水环境重金属污染的指示动物，从而为长效监测、合理治理污染和定位修复洞穴水环境的重金属污染提供科学的依据，这对确保研究区域内的人群健康和农作物净化均具有重要的理论和实践意义。

this project will mainly investigate the following four questions: 1) the distribution characteristics of heavy metals in Karst caves and their risk on human health, 2) the distribution characteristics, bioaccumulation and health risk assessment of heavy metals in the edible aquatic animals in Karst caves, 3) indicator species of aquatic animals in Karst caves, 4) the content of isotope Pb in water bodies of Karst caves and to reveal the source of pollutants and the contribution rate of partial study objects by tracing the fingerprints of isotope Pb in automobile exhaust, coal burning, industrial emission and pesticide application in neighboring areas of Karst caves. This project will find out the relative abundance and species composition (population) of aquatic animals in Karst caves in Guizhou province, the status of heavy metal pollution of water body and aquatic animals in Karst caves as well as their risk on human health, the source of pollutants in the water environment of Karst caves and indicator animals of heavy metal pollution for water environment of Karst caves. Our results will supply scientific basis to long-acting monitoring, reasonable pollution management and targeted restoration of heavy metal pollution of water environment in Karst caves, which also has an important theoretical and practical significance for human health and crop purification in the study area.