睑板腺功能障碍（Meibomian Gland Dysfunction，MGD）是眼科临床常见病和多发病，可引起持续性眼表面炎症，影响患者视觉功能和生活质量。迄今为止，MGD导致眼表面病变的病理生理过程还不清楚。本项目拟采用6种具有先天性睑板腺发育异常或后天性睑板腺萎缩的转基因鼠及基因突变鼠作为研究对象，筛选MGD眼表面病变的动物模型。通过对模型鼠睑板腺、泪液、眼表组织病理改变的观察，分析MGD导致眼表面病变的病理生理过程，并探讨炎症相关、细胞增殖及分化相关的信号通路在MGD眼表面病变发生发展过程中的激活情况。本项目的实施将加深我们对睑板腺分泌液生理功能的认识，有助于阐明MGD相关眼表面病变的分子机制，为MGD相关疾病的治疗研究提供新的实验平台。

Meibomian gland dysfunction (MGD) is an eye disease with high prevalence which can cause persistent ocular surface inflammation, and has effect on visual function and life quality. However, the pathophysiological process of MGD induced ocular surface abnormality remains elusive. In this project, we plan to use 6 lines transgenic mice or gene mutation mice which showed abnormal meibomian gland development or meibomian gland atrophy in adult stage to screen an animal model for MGD induced ocular surface abnormality. The pathologic change of meibomian gland, tear film, and ocular surface tissues such as cornea and conjunctiva will be investigated to determine the pathophysiological process of MGD induced ocular surface abnormality. Moreover, the activation of inflammation, cell proliferation and differentiation related signaling pathways will be evaluated to explore the function of these signaling pathways in the disease development. The implementation of this project will deepen our knowledge on the physiological function of meibum, shed new light on elucidation of the molecular mechanism of MGD related ocular surface disease, and provide new experimental platform for treatment research on MGD related diseases.