高甘油三酯血症性急性胰腺炎（hypertriglyceridemic pancreatitis, HTGP）的病情多呈重症化，发病机制尚需深入研究。前期实验发现，HTGP中胰腺的病理改变与sXBP-1的表达上调呈现相关性，但作用不明。近期国外研究发现，调控炎症反应的CCAAT增强子结合蛋白?（C/EBP?）的表达受内质网应激的IRE1α途径调控。本课题从两方面研究：（1）构建高甘油三酯血症急性胰腺炎动物模型，观察IRE1α/XBP-1途径中C/EBPβ相关分子的表达与胰腺形态、外分泌功能和早期炎症反应的相关性，证实经该途径调节的C/EBPβ参与了HTGP发病过程；（2）应用siRNA和shRNA下调C/EBPα，瞬转和稳转胰腺腺泡细胞，腺病毒表达载体表达上调C/EBP?，阐明C/EBPβ的表达是受sXBP-1-C/EBPα-C/EBPβ轴调控，为防治HTGP提供理伦依据。

The clinical course of hypertriglyceridemic pancreatitis (HTGP) is often more severe type and the exact machanisms involved in HTGP are unclear.In previous study,we found up-regulation of sXBP-1 is associated with HTGP,but the role is unknown.Recently,some foreign studies discovered C/EBPbeta in IRE1α/XBP-1 pathway which regulates the inflammation.Our study aim to detect ：（1）In the animal model of hypertriglyceridemic acute pancreatitis,we study the expressiosn of C/EBPbeta in IRE1α/XBP-1 pathway associated with the pathologic changes and exocrine function of pancreas and inflammation in early stage of HTGP.We hope to verify that C/EBP? in IRE1α/XBP-1 pathway participated in pathogenesis of HTGP.(2)By using siRNA and shRNA of C/EBPα and up-regulation of C/EBP?,we aim to confirm the expression of C/EBP? was regulated by axis of sXBP-1-C/EBPα-CEBP/?.The results of our study will provide a theoretical basis in prevention and therapy in HTGP.