杂环溴化阻燃剂- - 三-(2,3-二溴丙基）异氰脲酸酯（TBC）是溴代联苯醚类、四溴双酚A等多种常规溴化阻燃剂的替代产品，在国内已逐渐批量生产及应用，但它最近被证明是一种具有环境持久性的新型有机污染物，其潜在的环境危害和生态风险值得关注。本项目重点研究TBC抑制斑马鱼体内卵黄蛋白原合成、阻碍成鱼性腺发育的作用位点及途径，通过结合利用多种内分泌干扰物离体、活体生物测试模型，并计算TBC与雌激素受体的定量构效关系，以揭示TBC产生内分泌干扰效应的分子致毒机制；同时选择斑马鱼作为模式动物，进一步研究环境浓度下TBC长期低剂量暴露对斑马鱼生长、发育、繁殖及子代的毒性效应，着重探讨TBC是否通过干扰正常内分泌系统功能造成潜在生殖毒性及其具体的作用途径，最终为准确评估TBC这种新型有机污染物的生态风险和环境健康效应，并为溴化阻燃剂及其替代产品的生产和应用提供科学依据。

Heterocyclic brominated flame retardant tris-(2,3-dibromopropyl) isocyanurate(TBC) is the substitute of regular brominated flame retardants such as polybrominated diphenyl ethers(PBDEs) and tetrabromobisphenol A (TBBPA). It has been batch produced and used domestically. However, TBC is recently proven to be a new environmental persistent organic pollutant, and its potential environmental effects and ecological risk should be taken seriously. Our research focuses on the action sites and pathways though which TBC suppresses hepatic vitellogenin synthesis and hinders gonad development in adult zebrafish（Danio rerio）. By utilizing different in vitro and in vivo bioassay models of endocrine disrupting chemicals (EDCs) and calculating the quantitative structure activity relationship of TBC and estrogen receptor, the molecular mechanism of endocrine toxic effects caused by TBC can be revealed. Meanwhile, zebrafish will be exposed to low-doses of TBC according to environmental concentrations for a full lifetime, in order to study TBC's toxicity effects on growth, development, reproduction and offspring. Furthermore, whether TBC could cause reproductive toxicity through disrupting the functions of endocrine system and the precise mechanism will be discussed. These results can be provided as scientific evidences for evaluating the ecosystem risk and environmental health effects caused by TBC, as well as guiding for production and application of brominated flame retardants and its substitutes.