近年来随着免疫性疾病的增长，脊柱结核发病率逐年增加，但脊柱结核椎间盘的破坏机制仍然不清。我们的初期研究发现在脊柱结核椎间盘破坏过程中，微小RNA（microRNA）和基质金属蛋白酶（MMP-8、-9、-13）发挥着重要的作用，微小RNA可能影响基质金属蛋白酶的表达。本研究以脊柱结核患者病灶部位椎间盘为研究对象,应用生物信息学结合基因过表达及基因抑制、RT-PCR、Western-blot、Annexin V-FITC染色法、流式细胞等分子生物技术，从MMP-8、9、13的一种重要的内源性调控模式即miR-155对靶基因MMP-8、9、13的调控方面着手，通过上调和抑制miR-155来分析验证其对MMP-8、9、13表达的调控作用，旨在明确MMP-8、9、13在体内表达调控的重要途径，为研究脊柱结核椎间盘破坏机制提供新的思路,为寻找脊柱结核治疗干预的新靶点提供实验基础

Tuberculosis presents a challenging worldwide public heath problem.In recent years with the growth of autoimmune diseases,spinal tuberculosis incidence increased year by year.Although spinal tuberculosis is common, very little information is available on the inflammatory and immune mechanisms that are involved in its development.Our initial findings found that the micro RNA and metalloprotease(MMP-8,-9 and -13)plays an important role in destruction process of disc,micro RNA probably influence the expression of matrix metalloproteinases.In this study,bioinformatics,gene overexpression,gene inhibition,RT-PCR,western-blot,Annexin V-FITC staining,flow cytometry and other molecular biology technology, through the patients with spinal tuberculosis lesion as the research object.Up-regulation and inhibition of miR-155 to analyze the regulation mechanism of expression of MMP-8,-9 and-13.The study is to provide new ideas for spinal tuberculosis disc destructive mechanisms,and search new targets for therapeutic intervention in spinal tuberculosis.