近年来随着免疫性疾病的增长，脊柱结核发病率逐年增加，但脊柱结核椎间盘的破坏机制仍然不清。我们的初期研究发现在脊柱结核椎间盘破坏过程中，miR-155调控基质金属蛋白酶（MMP-8、-9、-13）的释放并且与lnc-XLOC表达相关。本项目将ceRNA的概念引入到miR-155在椎间盘破坏的调控机制中，拟综合运用生物信息学预测、qRT-PCR、Pulldown、Northern blot及荧光素酶报告基因系统等研究技术，分析lnc-XLOC作为ceRNA对mir-155调控作用,明确lnc-XLOC的调控机制，为研究脊柱结核椎间盘破坏机制提供新的思路,为寻找脊柱结核治疗干预的新靶点提供实验基础

In recent years, with the growth of immune diseases, the incidence of spinal tuberculosis increased year by year, but the destruction mechanism of spinal tuberculosis disc is still unclear. Our initial study found that miR-155 regulates the release of matrix metalloproteinases (MMP-8, -9, -13) during spinal disc disruption. In this project, the concept of ceRNA was introduced into the regulation mechanism of mir-155 in the destruction of intervertebral disc. The research of lnc-RNA was carried out by using bioinformatics prediction, qRT-PCR,Pulldown,Northern blot and luciferase reporter gene system. To investigate the effect of lnc-XLOC on the regulation of mir-155, to clarify the regulation mechanism of lnc-XLOC, to provide a new idea for studying the mechanism of spinal disc disruption, and to provide experimental basis for finding new targets for treatment of spinal tuberculosis