本课题以奶山羊乳腺上皮细胞为研究对象，采用显微观察、Western blot、RT-qPCR、CCK、免疫荧光、流式细胞仪、转录组测序、气相色谱-质谱联用等技术，分析羊表皮生长因子受体（EGFR）基因生物学特性；检测EGFR基因对羊乳腺上皮细胞结构、细胞增殖及细胞周期的影响。提取乳腺上皮细胞总RNA，分析在EGFR基因超表达或沉默后乳腺上皮细胞转录组变化。借用EGF、AG1478、LY294002、U0126、U73122，分析EGFR蛋白介导的MAPK、PI3K/akt、PLC-γ1信号通路活性及其相互关系，进一步研究EGFR调控甘油三酯及乳铁蛋白合成的分子途径。该研究将从理论上明确EGFR通过所介导的特异信号通路以调控乳脂及乳蛋白代谢，获得的研究成果将对于提高羊乳品质以及培育优质奶山羊品种等提供新思路。

The project is going through the methods (Microexamination, Western blot, Real-time quantitative PCR, CCK, Immunofluorescence, Flow cytometry, transcriptome sequencing, gas chromatography-mass spectrometry and so on) with goat mammary gland epithelial cells as experimental object. To begin with, the biological characteristics of epidermal growth factor receptor (EGFR) gene are analyzed. Then, the effects of EGFR gene on cell structure, proliferation and cycle of dairy goat mammary gland epithelial cells are detected. Total mRNAs of goat mammary gland epithelial cells with EGFR gene overexpression or silencing are extracted to analyze the transcriptome changes. Employing EGF, AG1478, LY294002, U0126 and U73122, the activities and relationships of MAPK, PI3K/akt and PLC-γ1 pathways in mammary gland epithelial cells are investigated. And furthermore, the molecular pathways of EGFR regulating the synthesis of triacylglycerol and lactoferrin are studied. The research will certify the regulation mechanisms of EGFR-mediated signaling pathways to milk fat and proteins in theory, and the results of which provide new considerations for improving milk quality and dairy goat breeding and so on.