山羊卵泡选择过程候选LncRNA作用机制研究

卵泡选择是决定排卵数量的关键步骤，生理过程复杂，目前卵泡选择的机制尚未明确。探索卵泡选择中长链非编码RNA（LncRNA）的调控机制，对山羊排卵机制研究和卵泡资源的储备开发都具有重要意义。本项目根据前期利用高通量测序技术，在不同发育阶段卵泡筛选出的特异表达LncRNA，并初步鉴定出可能与卵泡选择发育所需能量来源的线粒体氧化磷酸化作用相关的3个候选LncRNAs的基础上，利用RNA干扰、真核表达结合氧活性检测、激素水平检测、多分子水平检测等手段，进一步筛选候选LncRNAs与靶基因，明确二者在山羊卵泡选择过程中的作用机制。

Follicles selection is a key step to determine the number of ovulated oocytes. Until now, this mechanism is unclear due to its complexity of the physiological process. It is critical to the understanding of follicle selection mechanism by studying the involvement and regulation of lncRNAs in goat follicular selection..According to our preliminary screening for differently expressed LncRNAs at different development stages of the follicles via high-throughput sequencing technologies, three candidate LncRNAs related to mitochondrial oxidative phosphorylation were identified..In this project, these three candidate LncRNAs will be considered as specific targets to explain how these candidate LncRNAs and related genes utilize oxidative phosphorylation to regulate the selection process of follicles using RNA interference and eukaryotic expression technology platform combined with tests for changes in oxygen activity and hormones among other biomarkers.