大气细颗粒物中类腐殖酸类化合物的有效毒性组分的筛选和鉴定

大气细颗粒物中有效毒性组分的筛选和鉴定是环境大气化学领域的关键科学问题。最新的研究发现，细颗粒物中有机碳的主要成分类腐殖酸类化合物（Humic-like Substances HULIS）对人体心肺健康有显著危害，但是由于近期才被关注，HULIS诱导的毒性特点，及有效毒性组分的结构、组成和来源特征尚缺乏系统研究。本研究基于前期对大气HULIS的毒性研究的基础，针对不同典型排放源PM2.5中的HULIS样品，利用人体肺细胞模型，分析其毒性差异和剂量效应关系，及其主要信号通路，并利用核磁共振（NMR），超高分辨质谱（FT-ICR MS），分析有效毒性组分的结构组成信息，结论在真实大气中做进一步验证，利用加速器质谱（AMS）等技术进行源解析，探索筛选HULIS中有效毒性组分的结构组成信息和源信息。结论可以为优化大气污染物的排放标准提供关键数据，并为大气颗粒物的精准治疗和预防提供精确靶点。

The screening of effective toxic components in fine particles is a key scientific problem in the field of atmospheric environmental chemistry. The latest research found that the harm of Humic-Like Substances (HULIS), the main component of organic carbon in fine particles, to human health cannot be ignored. But because of the recent attention, the toxicity characteristics induced by HULIS, and the structure, composition and source characteristics of effective toxic components are still lack of systematic research. Based on our previous study on the toxicity of HULIS, this study analyzed the toxicity difference, dose effect relationship and special pathway of HULIS in PM2.5 of different emission sources by using human lung cell model, what’s more, using Nuclear Magnetic Resonance（NMR）, Fourier-transform ion cyclotron resonance-mass spectrometer（FT-ICR MS）, and other technologies to analyze the structure and composition information of effective toxic components of HULIS. The results will be further analyzed and validated in the real atmospheric samples (HULIS samples of PM2.5 in Guangzhou urban and suburb during one year). The toxicity changes of HULIS in different seasons and regions and the differences of its signal pathways were analyzed. The source information of HULIS in Guangzhou urban and suburb was analyzed by accelerator mass spectrometry (AMS) to verify the contribution of different emission sources to toxicity. The structure and composition characteristics of the effect toxic components of HULIS was analyzed and identified. The conclusion of this project can provide key data for the establishment of emission standards of air pollutants, and provide accurate targets for the precise treatment and prevention of atmospheric particles.