基于荧光纳米金刚石的高精度磁镊装置及RNA聚合酶转录动力学的研究

批准号:
12004420
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
王爽
依托单位:
学科分类:
软凝聚态与生物物理
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
王爽
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中文摘要
单分子磁镊技术是一种力谱学方法,它可以模拟体内的力学环境,表征生物大分子的动力学过程,以及该过程的力和能量等动力学和热力学参数。磁镊的精度决定于施力大小,在小力下精度较低,无法满足许多生命过程微小变化的测量。本项目拟利用荧光纳米金刚石(Fluorescent Nanodiamond, FND)探针对原有全内反射增强磁镊装置优化改进,旨在改善磁镊小力下测量精度的不足,提高磁镊在小力下的测量精度和测量时间尺度,并将其应用在小力下的生命过程研究中。构建特殊DNA底物,在小力条件下,更温和地、高精度地、长时间尺度地研究RNA聚合酶的转录动力学,深化对基因转录动力学及相关调控机制的理解,推动生物和物理学科的共同发展。
英文摘要
Single-molecule magnetic trap is a force spectroscopy technique capable of mimicking the force conditions in cells and determining the kinetics of biomacromolecules and the involved force and energy parameters. The spatial resolution of a magnetic trap is determined by the applied force where small forces give low resolutions, and as a result, the trap cannot be used for nanoscale detections in life processes under small forces. In this project, we will take the advantages of the fluorescent nanodiamond (FND) to improve the spatial resolution and the measurement timescale of the Total-Internal-Reflection (TIR) illuminated magnetic trap and thus the trap would be suitable for the kinetic studies of life processes under small forces. By construction of special DNA substrates compatible with the trap, we will be able to more gently investigate the transcription kinetics of RNA polymerase with a high spatial resolution and a long measurement timescale under small forces. These results will deeper our interpretations of the kinetics and related regulations in gene expressions, and will also promote the developments of both biology and physics.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
DOI:10.1073/pnas.2300282120
发表时间:2023-05-30
期刊:PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子:11.1
作者:Shi, Jing;Feng, Zhenzhen;Xu, Juncao;Li, Fangfang;Zhang, Yuqiong;Wen, Aijia;Wang, Fulin;Song, Qian;Wang, Lu;Cui, Hong;Tong, Shujuan;Chen, Peiying;Zhu, Yejin;Zhao, Guoping;Wang, Shuang;Feng, Yu;Lin, Wei
通讯作者:Lin, Wei
Structural basis of three different transcription activation strategies adopted by a single regulator SoxS.
单一调节器 SoxS 采用的三种不同转录激活策略的结构基础
DOI:10.1093/nar/gkac898
发表时间:2022-10-28
期刊:NUCLEIC ACIDS RESEARCH
影响因子:14.9
作者:Shi, Jing;Wang, Lu;Wen, Aijia;Wang, Fulin;Zhang, Yuqiong;Yu, Libing;Li, Fangfang;Jin, Yuanling;Feng, Zhenzhen;Li, Jiacong;Yang, Yujiao;Gao, Fei;Zhang, Yu;Feng, Yu;Wang, Shuang;Zhao, Wei;Lin, Wei
通讯作者:Lin, Wei
DOI:10.1093/nar/gkac433
发表时间:2022-06-10
期刊:NUCLEIC ACIDS RESEARCH
影响因子:14.9
作者:Shi, Jing;Wang, Fulin;Li, Fangfang;Wang, Lu;Xiong, Ying;Wen, Aijia;Jin, Yuanling;Jin, Sha;Gao, Fei;Feng, Zhenzhen;Li, Jiacong;Zhang, Yu;Shang, Zhuo;Wang, Shuang;Feng, Yu;Lin, Wei
通讯作者:Lin, Wei
DOI:10.1021/acs.jpcb.2c08771
发表时间:2023
期刊:The Journal of Physical Chemistry B
影响因子:--
作者:Yuqiong Zhang;Weijing Han;Lisha Wang;Hao Wang;Qi Jia;Tongsheng Chen;Shuang Wang;Ming Li
通讯作者:Ming Li
DOI:10.7498/aps.72.20231089
发表时间:2023
期刊:物理学报
影响因子:--
作者:张志鹏;刘帅;张玉琼;熊影;韩伟静;陈同生;王爽
通讯作者:王爽
Sen1介导非编码RNA转录调控机制的单分子研究
- 批准号:--
- 项目类别:面上项目
- 资助金额:58万元
- 批准年份:2020
- 负责人:王爽
- 依托单位:
国内基金
海外基金
