COP9信号复合体亚基调控水稻抗稻瘟菌的分子机制研究

批准号:
32001858
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
王如意
依托单位:
学科分类:
植物病理学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
王如意
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中文摘要
稻瘟病是最严重的真菌病害之一,影响水稻产量和粮食安全。解析水稻抗病机制对开发病害防控策略具有重要意义。COP9信号复合体通过去NEDD化修饰调节Cullin-RING泛素连接酶的活性,进而调控生物学过程。本项目前期发现水稻泛素连接酶APIP10正调控稻瘟菌的抗性,但其底物蛋白以及信号途径还不清楚。酵母双杂交发现APIP10与COP9信号复合体亚基OsCSN5相互作用。超量表达OsCSN5显著增强了水稻对稻瘟菌的抗性。进一步研究表明OsCSN5与Cullin蛋白OsCUL3a存在相互作用。本项目拟创制OsCSN5的功能缺失突变体研究其抗病性,运用生物化学方法分析APIP10对OsCSN5的泛素化修饰,以及OsCSN5对OsCUL3a的去NEDD化修饰。解析APIP10-OsCSN5-OsCUL3a调控水稻抗病机制。研究为解析COP9信号复合体调控植物免疫反应机制和培育水稻抗病品种提供新思路。
英文摘要
Rice blast disease is one of the most devastating fungal diseases and a constant threat to rice production and food security. Deep understanding of the mechanism of disease resistance contributes to the development of new disease prevention and control strategies. The COP9 signalosome (CSN) is an evolutionary conserved multiprotein complex that mediates the Cullin-RING ubiquitin ligase activity through deneddylation of Cullin proteins to control many biological processes. Previous studies found that the RING-type E3 ubiquitin ligase APIP10 positively modulates resistance to the rice blast fungus Magnaporthe oryzae, however, its substrates and related signaling pathway are still poorly understood. We identified the APIP10-interacting protein OsCSN5, a critical subunit of the COP9 signalosome. Overexpression of OsCSN5 in rice significantly enhances the resistance to M. oryzae. Furthermore, we also found that the Cullin protein OsCUL3a can interact with OsCSN5 in vivo. In this project, we will generate oscsn5 lose-of-function mutants via CRISPR-Cas9 technology and evaluate their resistance to M. oryzae. We will also investigate the ubiquitination modification of OsCSN5 by APIP10, as well as the deneddylation of OsCUL3a by OsCSN5. Combining genetic approaches with biochemistry methods in analyzing their relationship in immunity signaling pathway, we will uncover the underlying immune mechanism of APIP10-OsCSN5 -OsCUL3a-mediated rice blast resistance. The new insights to be obtained in this project will not only lay the foundation to unravel the COP9 signalosome-modulated immunity, but also provide new strategies for breeding resistant rice varieties.
期刊论文列表
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科研奖励列表
会议论文列表
专利列表
DOI:--
发表时间:2022
期刊:Trends in plant science
影响因子:--
作者:Ruyi Wang;Xiao Xu;Guo-Liang Wang;Yuese Ning
通讯作者:Yuese Ning
DOI:10.1016/j.tim.2022.12.001
发表时间:2022-12
期刊:Trends in microbiology
影响因子:15.9
作者:Fan Zhang;Min Wang;Guo‐Liang Wang;Yuese Ning;Ruyi Wang
通讯作者:Fan Zhang;Min Wang;Guo‐Liang Wang;Yuese Ning;Ruyi Wang
DOI:10.1186/s13059-022-02717-8
发表时间:2022-07-11
期刊:Genome biology
影响因子:12.3
作者:
通讯作者:
DOI:https://doi.org/10.1007/s42994-023-00111-x
发表时间:2023
期刊:aBIOTECH
影响因子:--
作者:Ruyi Wang;Guo-Liang Wang;Yuese Ning
通讯作者:Yuese Ning
国内基金
海外基金
