高温胁迫是番茄生产上最严重的非生物胁迫因子之一；高温胁迫条件下，子房发育是否正常是决定番茄产量及商品性的最基本条件。前期我们通过转录组学和表达谱研究发现，在番茄子房应答高温胁迫过程中，小热激蛋白20（sHSP20）基因在耐热番茄中表达量比热敏感番茄上调近97倍。基于此，本研究将构建sHSP20基因过量表达载体，进行热敏感番茄转化，对转基因番茄植株进行生理、生化分析及耐热性鉴定，以阐述sHSP20基因在番茄子房应答高温胁迫过程中的调控机理。此外，番茄基因组测序已经完成，我们将以模式作物拟南芥、水稻的热激蛋白为靶蛋白，检索番茄基因组数据库，获得较完整的热激蛋白种类和数目，分析其起源与进化，为阐明热激蛋白的功能保守提供理论基础；综上所述，项目实施不仅为阐明番茄子房应答高温胁迫的调控机理提供依据，而且还将明确番茄热激蛋白在植物进化中的方式及地位；进而为番茄耐热品种的培育提供遗传工程材料和理论基础。

High temperature stress is one of the most serious abiotic stress factors damaging tomato production. Under high temperature stress, whether ovary development is normal or not is the most basic decision tomato production and commercial conditions. Previously, by the transcriptome and expression profile studies, we have found that expression level of a small heat shock protein gene (sHSP20-18) is nearly 97 times higher in heat resistant tomato than heat sensitive tomato in ovary in response to high temperature stress. Based on this, in order to explore sHSP20 genes in tomato ovary response regulation mechanism under high temperature stress, we will make sHSP20 gene expression vector, transfer heat sensitive tomato, perform physiological and biochemical analysis and heat resistant identification in the transgenic tomato plants in this research. In addition, the tomato genome sequencing has been completed, we will take heat shock protein from the model plant Arabidopsis thaliana and rice as a target protein, search the tomato genome database, get a complete number of heat shock proteins, analyze their origin and evolution, and further provides the theoretical basis for elucidating the conservative function of the heat shock protein. In short, the implementation of the project not only help to elucidate the regulation mechanism of tomato ovary response to heat stress, but will also elucidate evolutionary way and status of tomato heat shock proteins in plant evolution, further provide genetic engineering materials and theoretical basis for breeding heat resistant varieties of tomato.