肠屏障功能障碍被认为在溃疡性结肠炎的发病机制中起重要作用，且肠屏障功能的破坏被认为与肠道粘膜炎症反应以及多种细胞因子有关。IL-22是一种近年来新发现并被广泛研究的细胞因子，其在溃疡性结肠炎中的作用目前尚未达成一致。本项目拟通过构建DSS诱导的小鼠急性溃疡性结肠炎模型并予IL-22腹腔注射干预，使用ELISA、Western Blotting、免疫组化及测定血浆D-乳酸等实验方法探究IL-22在溃疡性结肠炎中的作用，并找出其与肠屏障之间的关系，揭示其在溃疡性结肠炎中的作用及对肠屏障的影响。

Intestinal barrier dysfunction play an important role in ulcerative colitis. The pathogenesis involved with the intestinal mucosal inflammation and a variety of cytokines.IL-22 is a newly discovered cytokines which has been widely researched ; however,the delicate mechanism of IL-22 in ulcerative colitis has not yet been defined. Here we attempt to evaluate the role of IL-22 in the development of dextran sulfate sodium (DSS)- induced experimental colitis and . Mice would be injected intraperitoneal with IL-22; ELISA, Western Blotting, Immunohistochemistry and determination of plasma D-lactic acid and other experimental methods would be performed to identify its relationship with intestinal barrier.