蜂胶黄酮 Pinobanksin-3-acetate（PB3A）是有很高研发价值的单体黄酮类活性物质。以往工作中，我们建立了PB3A提取制备工艺，开展了生物学活性研究，明确该药抑制肿瘤细胞增殖、细胞周期阻滞和诱导细胞凋亡等作用机理。近期，我们通过结直肠癌SW480细胞的PB3A干预及人类miRNA表达谱分析，筛选出该药作用特异的候选差异表达miRNA，并预测出该药对细胞周期、凋亡和信号转导网络的影响，尚需候选miRNA指标的验证及其潜在的靶基因功能等一系列研究。故本研究拟以PB3A干预结直肠癌SW480细胞，采用定量RT-PCR和免疫印迹等方法，验证候选miRNA指标的特异性和灵敏度，预测和分析潜在的靶基因表达水平和功能调控，明确PB3A作用与候选miRNA及靶基因表达调控网络和信号转导通路变化的关系，探讨该药作用机理和潜在靶点，为基于PB3A新药研发和临床应用提供依据。

Propolis flavonoids Pinobanksin-3-acetate (PB3A) is a monomer flavonoid with bioactivity of high research value. In previous works, we established an extraction method for PB3A, and studied the biological activity, and found out several significant mechanisms of this drug, such as the inhibition of tumor cell proliferation, cell cycle arrest and induction of apoptosis. Recently，we studied miRNA expression profile of SW480 colon cancer cells in response to treatment with PB3A，and identified candidate differentially expressed miRNAs，and predicted the impact of the drug on cell cycle, apoptosis and signal transduction network, but further study is needed to validate the specificity of candidate miRNAs and functions of their potential target genes. In this study, we will treat SW480 colon cancer cells with PB3A, and analyze the specificity and sensitivity of previously identified candidate miRNAs and the expression and funtion of predicted target genes using methods like qRT-PCR and immuno blotting, for establishing the miRNA profile and revealing the signaling pathways of target genes changed in response to treatment with PB3A. The goal of this study is to further understand mechanisms and potential targets of this drug, and provide evidence for the development of a PB3A-based novel drug and its clinical application.