GW182(P-body标志蛋白)是我们新近发现的参与HCV复制的重要宿主因子（Hou W, et al. Hepatology. 2011;54(4) Suppl:332A Abstract 2038）。本课题拟在我们新建的含有报告基因的HCV细胞培养系统JFH1-hmAG/Huh7.5.1 和JFH1-hRluc/Huh7.5.1中, 通过基因沉默和过度表达GW182蛋白、激光共聚焦显微镜活细胞成像和免疫共沉淀等方法，研究GW182对HCV 正链及负链RNA合成的影响，GW182蛋白与HCV病毒蛋白的共定位及相互作用，研究GW182蛋白与miR-122共同调节HCV复制的作用，阐明GW182蛋白在HCV复制中的作用机制，深入了解GW182蛋白参与病毒生活史的具体过程，为将来针对参与HCV复制的宿主因子的靶向治疗提供候选分子和奠定实验基础。

GW182 ,as a Processing body(P-body) marker, is a novel host cofactor essential for hepatitis C virus(HCV) replication(Hou W, et al. Hepatology. 2011;54(4) Suppl: 332A Abstract 2038).In this study, by using the methods including knocking down and overexpression of GW182 as well as living cell imaging and co-immunoprecipitation, we study the effects of HCV positive and negtive strand RNA replication, the colocalization and interaction of GW812 and HCV viral protein, the role of GW182 and miR-122 in fine-tuning HCV replication,to explore the possible mechanisms of GW182 involved in HCV replcation ,to further our understanding of the HCV life cycle, and to explore the possible application of GW182 as the putative target in anti-HCV treatment.