乳酸链球菌素（Nisin）是一种常用的食品天然保鲜剂，但其在使用中易受温度、pH、食品组分等影响而导致抑菌性降低。前期研究发现Nisin与羧甲基壳聚糖（CMCS）形成纳米纤维是其有效解决途径，但其可控构建及持续抑菌机制尚未明确。本项目拟以CMCS和Nisin为主要研究对象，通过分子对接、数值模拟结合高倍场发扫描电镜、同步辐射小角X光散射等方法系统研究聚电解质自组装结合静电纺丝技术构建核壳结构CMCS-Nisin纳米纤维的可控途径及机理；同时，考察核壳结构纳米纤维对Nisin的缓释途径、抑菌稳定性的影响规律，从而揭示核壳结构CMCS-Nisin纳米纤维的持续抑菌机制。本项目研究可为天然活性物质的稳定化基础研究提供新的思路与理论借鉴。

Nisin is a common food-grade natural preservative, but it is susceptible to temperature, pH, food components, etc., leading to antibacterial activity loss. In our previous studies, the formation of nanofibers by nisin and carboxymethyl chitosan (CMCS) is one of the effective approach to solve this problem, however, the mechanism controllable construction and continuous antibacterial activity of these nanofibers are not clear. The molecular docking, numerical simulation, scanning electron microscopy and synchrotron radiation small angle X-ray scattering are integrated to study the formation mechanisms of core-shell structures of CMCS-Nisin electrospun nanofibers in a manner of the combination of self-assemble and electrospun. Besides, the effects of the core-shell of CMCS-Nisin nanofibers on the slow-release pathway and antibacterial stability of nisin will be investigated, and the continuous antibacterial mechanism of the core-shell of CMCS-Nisin nanofibers will be revealed. This study provides new ideas and theoretical references for the research and development of the stabilization of natural active substances .