糖尿病肾病是糖尿病严重的微血管并发症之一，也是导致糖尿病患者死亡的主要原因。我们课题组前期发现海带中一个由岩藻糖、半乳糖组成的褐藻硫酸多糖组分GFS是海带肾脏保护作用的主要活性成分，对糖尿病肾病有突出疗效，但是对于GFS治疗糖尿病肾病的作用机制尚不明确。本项目将基于糖尿病肾病发生过程中多糖及糖复合物参与的两个关键环节，包括乙酰肝素酶过度表达导致蛋白尿的发生以及选择素介导的炎症反应；分别采用离体细胞模型和糖尿病肾病动物模型，研究GFS对肾脏乙酰肝素酶和选择素的表达及其效应的调控作用，分析GFS抗凝血与其肾脏保护作用的相关性，探寻GFS治疗糖尿病肾病的作用靶点，阐明其肾脏保护作用机制。为开发新型抗糖尿病肾病药物提供药理学依据。

Diabetic nephropathy (DN) is a severe microvascular complication of diabete, and is the leading cause of renal failure and mortality of diabetes. We previously found a sulfated polysaccharide GFS from Laminaria japonica, mainly composed of fucose and galactose, is the main renoprotective component of Kunbu. GFS showed excellent curative effect on diabetic nephropathy and renal failure. But its renoprtective mechanism remains unclear. In this study, the effects and mechanisms of GFS on diabetic nephropathy will be investigated, using in vitro glomerular epithelial cell and in vivo DN rats model, from the points of heparanase induced proteinuria and selectin induced inflammatory reactions, two key carbohydrate participated process during the development of diabetic nephropathy. Based on carbohydrate interactions, the targets and renoprotective mechanisms of GFS on diabetic nephropathy will be explored and elucidated in this study. This study will provide a theoretical basis for the development of new durg candidate for diabetic nephropathy.