免疫炎症反应贯穿于同型半胱氨酸(Hcy)致病的全过程，前期研究表明miRNAs是Hcy致病的重要机制，但其受何种因素及如何调控仍未清楚。lncRNA作为新调控因子与miRNAs在结构上具有一致性，在调节方式上具有相似性，因此探讨两者相互作用方式成为焦点；而Rap1A是调控免疫炎症的重要基因，故推测：lncRNA与miRNAs通过“海绵吸附”作用调控Rap1A是Hcy致免疫炎症的重要机制。为了验证该假说，运用基因芯片筛选并确定LNC00657与miR-144是Hcy致免疫炎症的特异性lncRNA和miRNAs；采用免疫共沉淀和生物素特异性探针分别沉淀或下拉与miR-144和LNC00657结合的复合物，明确两者结合关系；构建miR-144生物传感器荧光素酶报告质粒，并与两者过表达质粒和/或共转染，分析Rap1A的变化，揭示两者相互作用调控Rap1A致免疫炎症的机制，为防治Hcy提供实验依据。

Immuneinflammatory through the whole pathogenic process of homocysteine (Hcy). Previous studies have shown that miRNAs involved in the pathogenic regulation of Hcy, but the mechanism is not clear. LncRNA as a new regulatory factor, it has the consistency with miRNAs in the structure, has similarity in the regulating mode, so the interaction between the two becomes the focus of research. Rap1A is an important gene which regulates immuneinflammatory response, so it can be assumed that lncRNA interact with miRNAs to regulate Rap1A is an important mechanism in Hcy-induced immune and inflammatory. In order to verify this hypothesis, using gene chip to screen and identify that LNC00657 and miR-144 are the specific lncRNA and miRNAs in Hcy-induced immuneinflammatory. Using immune coprecipitation and biotin specific probe respectively precipitation or drop-down the compounds combined with miR-144 and LNC00657, clear the combination relations. Construct miR-144 and LNC00657 biosensor luciferase report plasmid, and with both expression plasmid and/or transfection respectively, analyze the change of the Rap1A, reveal the mechanisms of the interaction between the two to regulate immune inflammation induced by Rap1A, which provides the experimental basis for the prevention and treatment of Hcy.