作为模式动物，七鳃鳗是研究适应性免疫起源和进化的重要实验材料。高迁移率族蛋白B1(high mobility group box 1,HMGB1)在人类淋巴细胞的分化发育中发挥重要作用。申请人前期工作发现，七鳃鳗HMGB1在淋巴造血组织和外周血中含有独特的类淋巴细胞中高表达，当利用脂多糖（LPS）和刀豆蛋白（ConA）免疫七鳃鳗使HMGB1基因表达量显著上调，说明七鳃鳗HMGB1与适应性免疫应答密切相关。本项目在分离培养七鳃鳗类淋巴细胞的基础上，运用共聚焦显微镜、酶联免疫吸附试验、荧光定量-PCR、流式细胞术及Transwell迁移等方法，探讨七鳃鳗HMGB1介导的类淋巴细胞的促炎，增殖及迁移作用，不仅为研究HMGB1蛋白在七鳃鳗独特的适应性免疫应答及炎症反应的作用机理提供线索，而且为鱼类疾病研究提供新的思路。

As a model organism, lamprey is important experimental materials on researching origin and evolution of the adaptive immune. The high mobility group box 1 plays an important role in human lymphocyte differentiation and development. Our previous research found that HMGB1 was expressed at a higher level in lymphocyte-like cells of lympho-hematopoietic tissue and peripheral blood. HMGB1 expression showed a significant up-regulation in the lymphocyte-like cells of the lamprey challenged with LPS and ConA. It was showed that lamprey HMGB1 correlated closely to adaptive immune response. The project study the role of lamprey HMGB1 in the lymphocytes proinflammatory, proliferation and migration using confocal microscopy,enzyme-linked immunosorbent assay, fluorescence quantitative PCR, flow cytometry and Transwell migration method on the basis of isolation and culture of lamprey lymphocytes, which can provide clues for the study of HMGB1 protein in lamprey unique adaptive immune responses and inflammatory reaction mechanism, in addition, provide new ideas for the study of fish disease.