Identification and characterization of the lamprey high-mobility group box 1 gene.
Identification and characterization of the lamprey high-mobility group box 1 gene.
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七鳃鳗高迁移率 Group Box 1 基因的鉴定和表征
DOI：
10.1371/journal.pone.0035755
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Pang Y;Xiao R;Liu X;Li Q
High-mobility group box 1 (HMGB1), a highly conserved DNA-binding protein, plays an important role in maintaining nucleosome structures, transcription, and inflammation. We identified a homolog of HMGB1 in the Japanese lamprey (Lampetra japonica). The Lampetra japonica HMGB1 gene (Lj-HMGB1) has over 70% sequence identity with its homologs in jawed vertebrates. Despite the reasonably high sequence identity with other HMGB1 proteins, Lj-HMGB1 did not group together with these proteins in a phylogenetic analysis. We examined Lj-HMGB1 expression in lymphocyte-like cells, and the kidneys, heart, gills, and intestines of lampreys before and after the animals were challenged with lipopolysaccharide (LPS) and concanavalin A (ConA). Lj-HMGB1 was initially expressed at a higher level in the heart, but after treatment with LPS and ConA only the gills demonstrated a significant up-regulation of expression. The recombinant Lj-HMGB1 (rLj-HMGB1) protein bound double-stranded DNA and induced the proliferation of human adenocarcinoma cells to a similar extent as human HMGB1. We further revealed that Lj-HMGB1 was able to induce the production of tumor necrosis factor-α (TNF-α), a pro-inflammatory mediator, in activated human acute monocytic leukemia cells. These results suggest that lampreys use HMGB1 to activate their innate immunity for the purpose of pathogen defense.
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影响因子：
64.8
作者：
Guo, Peng;Hirano, Masayuki;Herrin, Brantley R.;Li, Jianxu;Yu, Cuiling;Sadlonova, Andrea;Cooper, Max D.
通讯作者：
Cooper, Max D.
影响因子：
64.8
作者：
Pancer, Z;Amemiya, CT;Cooper, MD
通讯作者：
Cooper, MD
影响因子：
3.9
作者：
Bassi, Rosaria;Giussani, Paola;Riboni, Laura
通讯作者：
Riboni, Laura
影响因子：
6.7
作者：
Craig, Darren G. N.;Lee, Patricia;Simpson, Kenneth J.
通讯作者：
Simpson, Kenneth J.
影响因子：
7.4
作者：
Chung HW;Lee SG;Kim H;Hong DJ;Chung JB;Stroncek D;Lim JB
通讯作者：
Lim JB