I型真菌疏水蛋白由于其高效的固定化和分散活性，已成为倍受关注的新型生物纳米材料。但由于这类蛋白溶解度低，无法得到晶体结构，导致其发挥生物学功能的分子机制不清楚，使得I型真菌疏水蛋白在应用于药物缓释、生物传感器电极修饰等过程中环境因素的确定缺乏有力科学依据。. 本项目以I型真菌型疏水蛋白rHGFI为研究对象，选择其独特的杆状结构为突破口，拟通过荧光探针标记技术及圆二色谱、X射线光电子能谱和电镜等手段，对I型真菌疏水蛋白杆状结构的形成条件、结构组成变化及其与成膜稳定性和生物学功能之间的关系进行研究。找到制约I型真菌疏水蛋白杆状结构形成的关键因素；阐明杆状结构形成过程中单体分子内部、分子间结构变化以及相互缔合的作用机理，进而揭示I型真菌疏水蛋白杆状结构与其成膜稳定性、发挥分散固定化作用关系的分子机理和分子间识别的结构理论，为更广泛地研究和应用疏水蛋白作为生物纳米材料提供理论依据。

Based on their immobilization and dispersing activity, the class I hydrophobins are considered as interesting candidates for biological nanometermaterial. However, due to the low solubility of these proteins, the crystal structure can not be obtained, and the relationship of its protein structure and biological function is unclear, so that the determination of condition in the process of drug release and biosensor electrode modification is lack of strong scientific basis..In this project, the recombinant class I hydrophobin HGFI (rHGFI) is employed as the target protein, and the rodlets structure of this protein is chosen as breakthrough. The aim of this study is to explore the formation conditions, structure changes of the class I hydrophobin rodlets structure and the correlation between the rodlets structure formation, changes and the film stability and biological functions, which could be analysed through fluorescence probe technique, circular dichroism spectrum, electron microscope and so on. Meanwhile, we also expect to find the key factors that restricted the rodlets structure formation of the class I hydrophobin and to illuminate the structural changes of monomer intramolecular and intermolecular and their association mechanism. This will reveal the relationship between the hydrophobin rodlets and the structural theory of the membrane stability, molecular mechanism and intermolecular recognition in disperse and immobilized process. This will provide theoretical basis for research and application of hydrophobin used as biological nanometermaterial.