纳米颗粒在磷脂层的界面过程直接关系到其在生物体内的传输和应用安全性，但纳米颗粒的作用机制及引起磷脂层变化的规律尚待研究。本研究选取氧化物（Al2O3、SiO2和TiO2）和碳基 (C60、单壁碳管和多壁碳管) 纳米颗粒，综合运用荧光分子探针、冷冻电镜（Cryo-TEM）、小角X-射线散射（SAXS）、全反射红外（ATR-FTIR）和固体核磁共振（ssNMR）等手段，分析纳米颗粒与磷脂分子间的相互作用，以及对磷脂层结构完整性、层内流动性和分子排布的影响。目的在于揭示纳米颗粒在磷脂层的界面过程，进而阐明纳米颗粒表面特性（材质、粒径、电荷、修饰等），和颗粒-磷脂分子间作用力（静电力、范德华力、氢键和疏水作用等）与磷脂层结构相态变化的关系。本研究将为现有纳米颗粒的安全性评价，以及未来新型纳米材料的设计和应用提供重要的科学依据。

The processes of nanoparticles on phospholipid bilayers relate to its transport and safety to organisms. Additional research work is required to address the mechanisms and rules of nanoparticle-induced bilayer changes. In this study, oxide (Al2O3, SiO2 and TiO2) and carbon-based (C60, single-walled carbon nanotube, and multi-walled carbon nanotube) nanoparticles will be selected to explore the interactions of nanoparticles with phospholipids, and the effects on the integrity, fluidity and lateral organization of bilayers. The techniques we will use include molecular fluorescent probes, cryo-TEM, small angle X-ray scattering, ATR-FTIR and solid-state NMR. The objectives are to clarify the processes of nanoparticles on phospholipid bilayers, and to understand the roles of particle surface properties (material, particle size, charge, surface decoration) and intermolecular forces (electrostatic forces, van der Waals forces, hydrogen bonds and hydrophobic effects) on the changes of bilayer phases and structures. This study will provide important scientific information on the safety evaluation of the existing nanoparticles, also on the design/application of new types of nanoparticles in the future.