在纳米颗粒表面重复呈现抗原，能够模拟天然微生物侵染过程，有效提升亚单位疫苗的效价。本研究前期自行设计了一种自组装纳米颗粒蛋白单体序列，并成功利用细菌O-糖基化系统实现了病原细菌多糖纳米颗粒的构建。本研究拟在此基础上，重点对自组装糖蛋白颗粒与免疫系统之间的相互作用这一科学问题进行详细研究：分析多糖纳米颗粒对树突状细胞的激活及其表面受体分子；明确T细胞增殖效率及疫苗注射后的体内分布。力争通过本项目研究，在抗感染纳米疫苗研究领域内获得先发优势，为我国病原细菌防控提供新型有效的备选方案和技术储备。

Repetitive antigen displays on the surface of nanoparticles can simulate the process of natural microbial infection and effectively increase the immune responses against subunit vaccines. In our previous study, a self-assembly protein sequence was designed, and nanoparticles modified by the polysaccharide of pathogenic bacteria were successfully constructed by the bacterial O-glycosylation system. Based on these results, this study focuses on the interaction between the self-assembled glycoprotein particles and the immune system: To analyze the activation of polysaccharide nanoparticles on dendritic cells and discover the surface receptor molecules for glycoprotein particles; To evaluate the T cell proliferation efficiency and the vaccine distribution after injection in vivo. Through this project, we want to obtain the first-move advantage in the field of anti-infective nano-vaccine, and to provide a new effective alternative for prevention and control of pathogenic bacteria in our country.