小麦条锈病是世界范围内小麦上最重要病害之一，在我国已发生6-7次大区域的流行，造成重大经济损失。利用高温持久抗病基因培育抗条锈病小麦品种是控制小麦条锈病的有效策略。开发专化性的分子标记，采用分子辅助选育是加速培育抗病品种的关键，也是克隆基因研究该类抗病机制的前提。本项目利用前期已经定位和作图的小偃6号中高温抗条锈基因Yrxyh的研究结果，筛选铭贤169/小偃6号杂交F2代5000个单株中的重组植株来构建精细作图群体，并采用ISSR、ISBP、DArT、SNP以及小麦染色体特异区域EST、小麦3B染色体物理图谱等标记，以及小麦-禾谷作物比较基因组学等生物信息学技术，构建小偃6号高温持久抗锈基因Yrxyh的精细高密度分子图谱。如有需要将采用染色体步移进一步逼近基因位点，开发可用于多个小麦遗传背景下的高温抗条锈基因的专化性分子标记。

Wheat stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most devastating diseases that plagues wheat production globally, especially in China. Six to seven nationwide stripe rust epidemics have been recorded in China since 1950, which resulted to large economic loss. Growing cultivars with high-temperature durable resistance genes is an effective approach to control the disease. Development of specialized molecular markers is essential for marker-assisted selection. It also sets the platform for further cloning the gene and studying the resistance mechanism. In our preliminary study, the high-temperature resistance gene in Xiaoyan 6 has been located on wheat chromosome 3BL. This research will construct the fine-mapping population by screening recombinant plants in 5000 F2 individual plants from the cross Mingxian 169/Xiaoyan 6. Using SSR, ISBP, DArT, SNP, EST and markers from wheat chromosome 3B physical map, and wheat-cereal crop comparative genomics and bioinformatics, a high-density map will be constructed. Chromosome walking will be employed to further narrow down the region if needed. Effective and reliable molecular markers will be developed for marker-assisted selection.