鼻中隔软骨（NSC）和下颌髁突软骨（MCC）作为颌面部重要的生长区，鼻中隔软骨不发生骨化，而髁突软骨可以成骨。本课题组发现FLRT2是引起这两种软骨形成差异的一个重要因素，而磁场可以影响NSC与MCC的增殖和分化；还发现FLRT2在胚胎面中部和下颌胚基中的表达程度不同，而且FLRT3和骨形成蛋白在下颌胚基中的表达有重叠。据此，我们推测FLRT2和/或FLRT3可能是引起鼻中隔软骨和髁突软骨成骨差异的一个重要因素，而静磁场可以影响这两种软骨的成骨。本研究拟通过动物实验和细胞实验，观察FLRT2,3在大鼠胚胎期和出生后NSC和MCC与软骨成骨的关系；探讨静磁场对鼻中隔软骨和髁突软骨成骨的影响；过表达和/或敲减FLRT2,3来研究FLRT2,3调控鼻中隔软骨和髁突软骨成骨差异的作用机制，结果将为深入了解颅颌面部软骨的生长发育机制，说明磁性材料的生物学作用，特别是为颅颌面畸形的防治提供新思路。

Nasal septal cartilage(NSC) and mandibular condylar cartilage(MCC) are important growth areas in the maxillofacial region. NSC does not get osteogenesis but MCC does. Previous study found that FLRT2 plays an important role in NSC and MCC, and the magnetic field can affect the proliferation and differentiation of NSC and MCC. The study also show that Flrt2 exhibit various expression on the craniofacial region and mandibular primordial of the embryo. Flrt3 is expressed in overlapping expression pattern with Bmp4. Accordingly, we hypothesize that FLRT2 and/or FLRT3 may be an important factor in osteogenesis of NSC and MCC, which can also be affected by the static magnetic field. The purpose of this study is to investigate the relationship between cartilages osteogenesis and FLRT2,3 from embryo to postnatal rats model and cell culture in vitro. To elucidate the effects of static magnetic field on osteogenesis of NSC and MCC. To explore the regulation of FLRT2,3 on osteogenesis mechanism by over expression or knock down FLRT2,3. In conclusion, this study will be helpful in further understanding of the growth and development mechanism of craniofacial cartilage, and explain the biological effect of magnetic materials, and particularly provide new strategies for the craniofacial deformity prevention and treatment.