鼻中隔软骨（NSC）作为原发性软骨、面中份的生长中心，下颌髁突软骨（MCC）作为继发性软骨、下颌的生长区，二者的软骨形成分化异常将引起颅颌面畸形。FLRT2是富亮氨酸跨膜纤维连接蛋白家族的第二成员。本课题组首次发现Flrt2在胚胎面中份和下颌胚基中有不同程度的明显表达，在软骨祖细胞株的软骨分化过程中促进细胞的增殖与分化。研究显示适当的磁场可以促进软骨的生长改建。因此，我们推测FLRT2在静磁场作中对NSC和MCC的形成分化起着重要的作用。本课题在建立静磁场加载NSC和MCC细胞培养模型的基础上，拟观察静磁场对NSC和MCC细胞分化的影响；检测FLRT2在静磁场中对NSC和MCC细胞分化的作用；通过敲减和过表达Flrt2，研究FLRT2在静磁场中调控NSC和MCC形成分化的作用机制。此研究将深入揭示颅颌面部软骨的生长发育，奠定磁性材料的临床应用基础，并为颅颌面畸形的防治提供新思路。

Nasal septum cartilage(NSC) is primary cartilage which is the growth center of the mid-facial area. Mandibular condylar cartilage(MCC) is secondary cartilage which is the growth site of mandible. The abnormal growth of NSC and/or MCC may lead to craniofacial deformity. FLRT2 is the second member of Fibronectin (F) Leucine (L) Rich (R)Transmembrane (T) family. Our research group first report that Flrt2 is highly expressed in the mid future chondrogenic nasal septal region and mandibular primordia. Flrt2 also can promote the proliferation and differentiation of ATDC5 cells during chondrogeneis. It is also reported that appropriate static magnetic field can increase the remodeling of condylar cartilage. So our hypotheses is FLRT2 play an important role during chondrogenesis in NSC and MCC under static magnetic field. We set up the cell culture model of NSC and MCC under static magnetic field to observe the effect of static magentic field to NSC and MCC during chondrogenesis, the effect of FLRT2 on chondrogenic process of NSC and MCC under static magnetic field and the role of FLRT2 under static magnetic field during the chondrogenic process of NSC and MCC after we knockdown and overexpress Flrt2. The research results will further describe craniofacial cartilage development and give the theoretical evidence for magnetic material clinical application. It will also provide a new idea to prevent and treat deformity of craniofacial development.