miR-30e-ATG5介导的自噬在DIM预防胃癌中的作用及机制

3,3'-二吲哚甲烷（DIM）是十字花科蔬菜的重要活性成分。针对其肿瘤预防作用已明确但机制尤其在胃癌中仍不清楚的研究现状，我们首次在胃癌中提出假说：自噬与DIM的胃癌预防作用可能有关，并拟进一步研究其机制。我们应用mRFP-GFP-LC3双荧光质粒转染，结合激光共聚焦显微镜观察发现，DIM对LC3II的上调作用可能是通过使自噬体清除受阻、自噬流下调来实现；利用si-RNA及商品化mimic手段，显示DIM的上述作用可能是通过对miR-30e-ATG5的调控实现。本项目拟进一步利用分子细胞生物学相关技术，结合动物模型，重点探讨miR-30e-ATG5介导的自噬在DIM预防胃癌中的作用及机制，以及miR-30e、ATG5与胃癌病理特征及预后的关联。本项目以自噬流为切入点，可对阐明DIM的肿瘤预防机制具有重要意义，并为胃癌的膳食预防及诊断预后提供思路。

Emerging evidence provide support in terms of the potential anticancer effect of bioactive products derived from cruciferous vegetables as DIM. Based on our previous study indicated that the cytotoxic effects were induced when DIM was treated in gastric cancer cells with LC3II up-regulated, we show that autophagy flux plays an important role in this effect using confocal microscopy after mRFP-GFP-LC3 transfection. Furthermore, we will use cellular, animal models with molecular and cellular biological techniques to identify the mechanisms of DIM through miR-30e-ATG5 axis in inhibiting proliferation of gastric cancer cells, which will provide new strategy as autophagy flux for preventing and treating gastric cancer.