蒜氨酸是大蒜的标志性含硫氨基酸，在蒜酶催化下产生活性物质大蒜辣素。硫化氢是发现的第三个信使分子，在神经、心血管等系统有重要作用。最新研究发现大蒜相关硫化物SAC，DADS，DATS等的活性作用是在体内产生H2S所致，而DADS，DATS均为大蒜辣素降解产物。我们推测蒜氨酸和大蒜辣素在体内可能也经产生H2S而发挥活性作用。本研究基于光纤传感结合微透析流动注射建立大鼠血液中H2S的在线检测方法；HPLC/MS/MS及HPLC柱前衍生化建立蒜氨酸与大蒜辣素含硫代谢物的测定方法；测定两种物质在血液中的蛋白结合率；通过研究蒜氨酸与CBS或CSE酶的作用、大蒜辣素与谷胱甘肽等的作用，阐述红细胞内外蒜氨酸与大蒜辣素的代谢途径及是否产生H2S。项目将从蒜氨酸和大蒜辣素在血液中代谢的角度阐明其与H2S介导的活性作用之间的关联性，为进一步研究蒜氨酸和大蒜辣素作为H2S供体的可能性提供依据。

Alliin is sulfur-containing marker of Garlic. Bioactive Allicin is produced by Allinase catalysis with Alliin. But in-vivo disposition and mechanism of Alliin and Allicin are unclear. Hydrogen sulfide is found the third gastransmiter and plays an important role in cardiovascular system. The latest research discovered that active effect of Garlic and Garlic-derivative compounds SAC, DADS, DATS were attribute to the release of H2S. We supposed that Alliin and Allicin exert active effects via releasing H2S. In this project, we will establish a microdialysis-flow injection analysis based on fiber-optic methods for on-line monitoring H2S in rat blood to diminish lose of H2S in traditionary sample treatment. HPLC/MS/MS and HPLC pre-column derivatization method will be set up for detecting the sulfur-containing metabolic intermediate of Alliin and Allicin. The protein binding ratio of Alliin and Allicin will be determined with assistant of microdialysis. In this study, we will investigate whether Alliin is the substrate of CBS or CSE, and whether Allicin can react with glutathione. From the possible metabolic pathway of Alliin and Allicin in rat blood and the releasing of H2S, we try to illustrate the relevance to bioactivity via hydrogen sulfide-mediated pathway. The supposed H2S donor of Alliin and Allicin will be inferred based on this research work.