听觉神经元声反应时程选择性普遍认为首先在下丘形成，其机制(“巧合模型”和/或“反巧合模型”)多基于对撤声反应神经元的研究，难以完全适用于给声反应神经元。本项目拟采用在体全细胞记录方式以不同钳制电压分离兴奋及抑制性输入，根据两者对不同声刺激时程的反应，建立小鼠下丘给声反应神经元声反应时程选择性形成机制模型。为完善该模型，我们拟利用局部微量注射γ-氨基丁酸和/或甘氨酸受体抑制剂及光遗传选择性抑制下丘γ-氨基丁酸能中间神经元，探查影响小鼠下丘给声反应神经元声反应时程选择性的抑制性输入的成分及来源。并通过在体全细胞记录条件下，研究不同成分及来源的抑制性输入参与小鼠下丘给声反应神经元声反应时程选择性形成的机制。

Duration tuning is widely thought being formed in the inferior colliculus (IC). The “coincidence” and “anti-coincidence” models are adopted to explain the mechanisms underlying the duration tuning formation majorly on off-response neurons. However, these models are not fully suitable for the on-response neurons. In this study, we firstly try to obtain the synaptic inputs an IC on-response neuron received to different sound duritions by adopting whole-cell recording technique and the excitatory and inhibitory inputs are separated by applying different holding potentials. Based on these results, we could develop a new duration tuning formation model suitable for on-response neurons. To identify the source and component of the inhibitory inputs involved in the duration tuning formation on the IC on-response neurons, we plan to study the effects of blocking the GABA and/or glycine receptors and selective inhibition of IC GABAergic interneurons using optogenetic technique on the inhibitory inputs an on-response neuron recieves to form duration tuning. These results would help us understand how the mice IC on-response neurons encode the stimulus duration and form the duration tuning.