外泌体miRNAs在PCa转移中发挥着重要作用，然而PCa转移相关miRNAs如何进入外泌体内却不清楚。在前期研究中我们通过RNA测序发现hsa_circ_0001922与PCa转移相关，并在PC-3M-1E8(高转移)中证实其表达显著高于低转移PC-3M-2B4，进而发现前者外泌体显著促进后者迁移，它们外泌体miRNAs表达差异明显，且PC-3M-1E8中hnRNPA1高表达。这些提示has_circ_0001922可能参与PCa外泌体miRNAs分选影响PCa转移，但其作用机制却不清楚。在本研究中，我们将通过PCa转移细胞模型分析hsa_circ_0001922调控外泌体miRNAs分选的分子机制，确认外泌体PCa转移相关miRNAs表达谱及其在PCa转移中的作用；进而通过裸鼠实验验证；最终通过病例-对照分析has_circ_0001922、外泌体miRNAs与PCa转移及预后的关系。

Exosomal miRNAs play an important role in the metastasis of PCa. However, it is unclear how miRNAs involved with PCa metastasis are loaded into exosomes. In the preliminary experiments, we found by transcriptome sequencing that hsa_circ_0001922 was associated with PCa metastasis, and confirmed that compared with PC-3M-2B4, the expression of hsa_circ_0001922 in high metastatic potential PC-3M-1E8 was increased significantly. In addition, we observed that exosomes from PC-3M-1E8 significantly promoted the migration potential of PC-3M-2B4, which involved with the different expressions of their exosomal miRNAs, and the high expression of hnRNPA1 was observed in PC-3M-1E8. These results indicate that has_circ_0001922 may affect PCa metastasis through regulating the sorting of exosomal miRNAs, but how the hsa_circ_0001922 works in the sorting of PCa exosomal miRNAs is unkown at present. In this study, we will use the PCa metastatic cell model to analyze the molecular mechanism of hsa_circ_0001922 regulating the sorting of exosomal miRNAs, confirm the expression profile of exosomal PCa metastasis-associated miRNAs and investigate their role in prostate cancer metastasis, and then validate the role of hsa_circ_0001922 in the PCa metastasis through nude mice experiments in vivo, and finally explore the relationship among has_circ_0001922, exosomal miRNAs and PCa metastasis and prognosis through a case-control study.