生物钟在植物生长、开花、抗性等性状调控中发挥着重要作用。大白菜是以营养期叶片为食用器官的重要蔬菜作物，全基因组信息的公布为开展器官发育等重要性状的功能基因组学研究提供了条件，但多数基因功能尚不明确，尤其是大白菜调控生长发育及代谢的生物钟相关基因的研究报道很少。本研究以同一遗传背景的大白菜生物钟长周期突变体及其野生型为材料，分析突变性状的遗传方式；利用Mutmap重测序和分子标记连锁分析，发掘影响大白菜生物钟节律延长的候选基因；利用RNA-seq基因表达谱技术，解析大白菜生物钟相关基因的表达调控模式；在建立大白菜CRISPR基因编辑技术基础上，结合真空渗透遗传转化，揭示生物钟关键基因在大白菜表达及性状调控中的功能。该项目对挖掘大白菜生物钟新型基因资源具有重要意义，也将为解析大白菜生物钟相关基因的分子互作提供理论依据，对揭秘生物钟调控大白菜昼夜节律、器官形成和生殖发育具有重要推动作用。

Circadian clock plays an important role in the regulation of plant growth, flowering and resistance. Chinese cabbage (Brassica rapa ssp. pekinensis) is one of important vegetable crops, which vegetative leaves are consumed as edible organs. Release of the whole genome information of Chinese cabbage provided important supports for functional genomics research on important traits such as organ development. However the function of most of genes is unclear and lacks of reports, especially the circadian clock related genes regulated growth development and metabolism in Chinese cabbage. In this study, the genetics of the mutant characters were analyzed by using the long-period circadian clock mutant (Lcc-1) and its wild type, with the same genetic background. By means of high-throughput sequencing Mutmap and molecular marker linkage analysis, the candidate genes that influence the prolonged circadian rhythm of Chinese cabbage will be discovered. Using RNA-seq technique, the expression regulation mode of the circadian clock related genes in Chinese cabbage was analyzed. On the basis of the establishment of CRISPR gene editing technology in Chinese cabbage, the function of the key genes related to the biological clock regulated gene expressions and agronomic characters will be revealed by combining the genetic transformation of vacuum infiltration. This project is of great significance to the mining new genetic resources of circadian clock in Chinese cabbage. And it will help analyze molecular interactions of circadian clock genes that provided the theoretical basis, and play an important role in revealing the regulations for circadian rhythm, organ formation and development by circadian clock in Chinese cabbage.