肾小球血管内皮细胞损伤是糖尿病肾病发生发展的重要机制之一。研究发现，microRNA-126可调控内皮细胞中VEGF及VCAM-1的表达，在血管内皮损伤中起着重要作用，我们前期研究也发现，microRNA-126在糖尿病肾病肾小球血管内皮细胞中表达下调，但其在糖尿病肾病发病中的作用尚不清楚。本项目拟采用载体构建、细胞转染、Western-Blot、qRT-PCR等方法，获得microRNA-126在糖尿病肾病内皮细胞损伤中的证据，阐明其作用机制。肾康丸从益气活血立法处方治疗糖尿病肾病在临床上取得良好疗效。我们前期研究发现肾康丸可调节microRNA的表达，并可有效抑制糖尿病肾病炎症反应，基于此结果，本研究将观察肾康丸在microRNA-126及其相关信号通路中的作用，以期丰富中药治疗的疗效机理，为糖尿病肾病的防治探索新的思路和方法。

Glomerular vascular endothelial cell injury is one of the important mechanism in the occurrence and development of diabetic nephropathy. It is showen that microRNA-126 plays an important role in the endothelial cell injury，which can regulate VEGF and VCAM-1 expression in the endothelial cells. Our previous study found that the expression of microRNA-126 was decreased in glomerular vascular endothelial cell.However, it is unclear what is the role of microRNA-126 in the pathogenesis of diabetic nephropathy. In order to get the evidence that microRNA-126 play an important role in the endothelial cell injury of diabetic nephropathy and clarify its mechanism, our research will use the methords, such as carrier building，cell transfection，Western-Blot，and qRT-PCR. Shen-Kang pill is prescribed in benefiting for activating blood circulation，and made good curative effect in clinical treatment of diabetic nephropathy. Our previous study found that Shen-Kang pill can regulate the expression of microRNA and effectively inhibit the inflammatory reaction of diabetic nephropathy. Based on the above result, our research will study the role of Shen-Kang pill in regulating microRNA-126 and its relative signal pathway. We expect that our research can enrich the traditional Chinese medicine treatment mechanism for diabetic nephropathy and explore new thought and method for the prevention and control of diabetic nephropathy.