甲状腺乳头状癌（PTC）是最常见的内分泌系统恶性肿瘤，肿瘤恶性程度存在显著异质性，目前仍缺乏有效的鉴别手段。本课题组通过生物信息学检索和组织标本验证发现MIR31HG在PTC中高表达并且与淋巴结转移和BRAF突变相关，进一步的细胞学实验发现PTC细胞株中MIR31HG核内分布比例异常增加。RNA pull down 实验发现MIR31HG与TTF1存在直接结合作用，TTF1是核糖体相关基因合成的重要调节因子，核糖体合成与肿瘤的侵袭性密切相关。因此本次研究的主要目的是揭示MIR31HG如何通过与TTF1蛋白结合调控PTC的发生发展。

Papillary thyroid cancer (PTC) is the most frequent malignant tumor of endocrine system, there are heterogeneity in the malignancy which is lack of detection method. Through bioinformatics and our tissue bank, we have found that MIR31HG overexpress and correlate with the lymph node metastasis in PTC, further in vitro study showed that there is unconventional increase of MIR31HG in the nuclear. RNA pull down experiment revealed that there was direct combination between MIR31HG and TTF1 (transcription termination factor). TTF1 is a key modulator of ribosome synthesize. The aim of this study is to find out how MIR31HG and TTF1 contribute to invasiveness of PTC.