汗腺对人体机能的协调和稳态是十分重要的，但汗腺的再生能力弱，使得临床上重度烧伤和创伤病人康复后的皮肤没有发汗功能，严重降低了其生活质量。目前主要通过干细胞和组织工程方法诱导汗腺再生，但工作量大，诱导效率低，缺乏对汗腺发生过程的完整描述，难以进行机制研究，更无法满足临床对汗腺功能再生的需求。微流控技术是组织工程领域的新兴技术，可以在微米尺度模拟复杂的生物学过程，由于微流控芯片中可以产生稳定的生物因子与材料层流梯度，因此在皮肤及其附属器的研究中有其先天优势，可以解决以上研究中遇到的问题。本研究旨在通过应用微流控技术精准构建汗腺的发育微环境，诱导汗腺组织形态发生，并对其生物学机制进行深入探索，以期建立高效诱导汗腺再生的体系；同时，构建含有汗腺组织的皮肤芯片，准确模拟正常皮肤的层状结构，以期构建皮肤和汗腺的发育和疾病的研究模型，为解决皮肤功能性再生（尤其是汗腺再生）的难题提供重要的理论基础。

Sweat glands are very important for the coordination and homeostasis of human body. But the regeneration ability of sweat glands is weak. Patients with severe burns and trauma have no sweating function after recovery, which seriously reduces their quality of life. At present, the regeneration of sweat glands is mainly induced by using stem cells and tissue engineering methods. However, due to the heavy workload, low induction efficiency and the lack of complete description of sweat glands morphogenesis, it is difficult to conduct mechanism research and to meet the clinical demand for functional regeneration of sweat glands. Microfluidic technology, which is a new technology in the field of tissue engineering, can simulate complex biological processes on the micron scale. As the microfluidic chip can produce stable biological factors and material gradient, it has inherent advantages in the research of skin and its appendices. Therefore, by using microfluidic technology, this research is aimed to accurately construct the microenvironment to induce morphogenesis of sweat glands, and to further explore its biological mechanism, so as to establish an efficient system for inducing the regeneration of sweat glands. Meanwhile, a skin-on-a-chip containing sweat gland tissue would be constructed to accurately simulate the layered structure of normal skin, so as to build a research model of the development and diseases of skin and sweat glands, and provide an important theoretical basis for solving the problem of functional skin regeneration (especially the regeneration of sweat glands).