氨基酸转运蛋白基因BnAAP3L参与生长素介导LBD29调控油菜侧根发育的分子机制研究

侧根占根系的大部分，对整个植株的生长发育极为重要。前期实验中，我们发现：过量表达甘蓝型油菜氨基酸转运蛋白BnAAP3L基因可增加转基因拟南芥的侧根数量及长度、生长素含量及植株高度等。进一步研究表明：一侧器官边界结构域蛋白LBD29基因的表达水平在该转基因植株中明显高于野生型对照。我们猜测：BnAAP3L参与了生长素介导的LBD29调控侧根的发育。本课题以甘蓝型油菜和拟南芥为研究材料，拟利用生物信息学、qPCR、转录组测序、转基因、报告基因显示、突变分析、分子杂交、显微及切片观察等技术方法，研究BnAAP3L基因参与生长素介导的LBD29信号传导途径调控侧根发育的分子机制。这将丰富并发展人们对氨基酸转运蛋白及侧根发育的认识，在农业基因工程中具有广泛的应用前景。

Lateral root occupies the majority of the root system, which is very important for the growth and development of the whole plant. In previous experiments, we found that over-expression of BnAAP3L, an amino acid transporter gene of Brassica napus, could enhance the number and length of lateral roots, auxin content and plant height of transgenic Arabidopsis thaliana. Further study showed that the expression level of one lateral organ boundaries domain-containing gene (LBD29) was significantly higher in the transgenic plant than that in the wild-type control. We hypothesize that BnAAP3L may participate in the auxin-mediated LBD29 regulation of lateral root development. This subject will use B. napus and A. thaliana as materials, and utilize the bioinformatics, qPCR, transcriptome sequencing, transgene, reporter gene display, mutation analysis, molecular hybridization, microscopic and slice observation, and other`s methods to investigate the molecular mechanism of BnAAP3L gene involved in the auxin-mediated LBD29 signaling pathway regulating lateral root development. This topic will enrich and develop people's understanding of the amino acid transporters and the lateral root development, which will have a wide application prospect in the genetic engineering of agriculture.