针对前哨淋巴结活检的特殊应用和检测分析要求，基于羧酸甜菜碱甲基丙烯酸甲酯（CBMA） 嵌段共聚物，构建新型的上转换发光/核磁共振成像（UCL/MR）多模式淋巴成像试剂，进行多模式淋巴成像研究。对CBMA 嵌段共聚物的嵌段结构和组成进行设计，通过共聚物嵌段链段在上转换发光纳米粒子表面对Gd3+进行螯合，实现多模式成像试剂的光学和磁学功能，并同时通过嵌段共聚物链段在纳米粒子和生物环境之间构建适当的生物界面，降低稀土元素在生物体内使用风险，提高成像试剂的生物有效性和相容性。以细胞生物学技术（MTT，流式细胞术、膜片钳）、计算机模拟手段与物理化学方法（原子力显微镜、透射电镜、上转换光学成像与磁共振成像技术）的优化组合为研究手段，研究CBMA 嵌段聚合物用于构筑新型多模式成像试剂的可能性，为淋巴成像提供有益的科研基础和借鉴。

Due to the special application of imaging for sentinel lymph node, new lymphatic imaging reagents will be designed for UCL/MR multimodal lymphatic imaging, based on a suitable CBMA block polymer. With the different CBMA block copolymer structure and composition, the novel lymphatic imaging reagents will be prepared for UCL/MR multimodal imaging by binding Gd3+ on the upconversion nanoparticles, which reduced the in-vivo risk on the application of rare earth , and the bio compatibility and effectiveness of will be checked in lymphoid tissue for UCL/MR multimodal lymphatic imaging. The research techniques include computer simulation, cell biology technology (MTT, patch clamp, flow cytometer) and physical and chemical method (atomic force microscope, transmission electron microscope, UCL imaging and magnetic resonance imaging technology); the promise of new imaging reagents with the CBMA block polymer will be discussed, and it will provide a useful scientific basis and reference for lymphatic imaging.