成像基元标记的高分子抗癌药物载体为癌症的可视化治疗提供了新工具。但是传统可视化高分子药物载体成像信号与药物释放过程无关或相关性差，只能利用成像信号定位高分子载体位置而无法追踪药物的释放过程。本项目拟通过控制合成技术，将基于“Turn-On”型荧光染料的还原敏感可视化小分子前药引入到结构精确，且电荷、尺寸和长径比可调的水溶性刷形高分子载体中，构筑刷形可视化高分子药物体系。拟系统研究该类体系在还原条件下荧光增强过程和药物释放过程的特定线性关系。利用细胞成像技术采集的荧光信号实现对该体系在细胞内药物释放过程的追踪检测。研究不同电荷、尺寸和长径比等因素对刷形高分子药物体系在癌症细胞中药物释放过程的影响规律，为新型可视化高分子药物体系的设计提供新的思路。

Imaging agents labeled theranostic polymeric anticancer drug delivery systems as new tools were widely used for the visualization of cancer treatment. However, the imaging signal and drug release process from traditional theranostic polymeric drug delivery systems were no specific relationships. Only polymeric vector positions could be determined by using the imaging signal. The drug release process from polymeric carriers could not be monitored. This project will synthesize a new class of well-defined water-soluble redox-responsive theranostic brush polymer drug conjugates (BPDCs) via controlled polymerization of the specially designed “Turn-On" fluorescent probes based redox-responsive theranostic prodrug monomers. The specific linear relationship between fluorescence enhancement process and drug release process of these theranostic BPDCs under redox conditions will be established. By taking these linear relationships, the intracellular drug release process from these these theranostic BPDCs in cancer cells would be tracked and quantified through the fluorescence signal by using the live-cell imaging fluorescence microscopy. Furthermore, the effects of surface charge, size and aspect ratio of the brush-shaped polymer drug carriers will be studied also. The project will provide a new strategy for design of next generation of theranostic polymeric drug carriers.