基于连锁不平衡的关联分析是目前发掘与鉴定优异基因的有效手段，可用于鉴定群体内目标性状与候选基因的关系。本研究以江西省境内抗坏血酸（AsA）含量具丰富变异的野生毛花猕猴桃(Actinidia eriantha Benth)种群为试验材料，利用覆盖猕猴桃全基因组的SSR标记，结合该野生群体AsA含量性状应用Tassel软件中的MLM进行全基因组关联分析，定位其AsA富集相关的主效QTLs。然后在主效QTLs区段内发掘调控AsA合成的关键酶GalDH、GalLDH和GalUR基因，通过基于候选基因的关联分析探究不同等位基因序列差异及其与AsA合成能力的关系，同时采用SYBR Green I实时荧光Q-PCR对其相关基因的表达水平进行分析，利用RACE技术获得其候选基因全长cDNA并进行遗传转化，解析候选基因的功能，探明其AsA合成相关的关键基因，从基因水平揭示野生毛花猕猴桃AsA富集的分子特征。

Recently, association analysis is a procedure for explore and identification excellent genes based on linkage disequilibrium (LD). Association analysis is an approach to identify the relationship of candidate genes with traits in a given population. This study intends to take the germplasm populations with rich variation of ascorbic acid (AsA) content in wild kiwifruit (Actinidia eriantha Benth) fruit in jiangxi province as experimental material. Association analysis between SSR loci and AsA content trait was performed using TASSEL MLM (mixed linear model) program. In the main effect QTLs segment screening and discover regulation AsA synthesis of key enzyme -GalDH、GalLDH、GalUR purpose gene through the analysis of candidate genes associated with wild kiwifruit (A. eriantha Benth) GalDH、GalLDH and GalUR gene sequence differences and their relationship with AsA synthesis ability. At the same time, the related genes cDNA will be systemetically analized by SYBR Green I real-time quantitative PCR during the expression level of different development periods.The cDNA of candidate genes will be obtained through RACE technology, and their function will be analyzed by gene transformation as well as the key genes related to rich AsA accumulation in wild kiwifrui could be found, which could receal the molecular character of AsA accumulation in wild kiwifruit from the gene level.