Ⅲ型前列腺炎（CP/CPPS）是最常见的前列腺疾病。前期通过iTRAQ 标记结合 2DLC-MALDI-TOF的质谱实验筛选出在CP/CPPS血清中高表达的蛋白SOD3，大样本ELISA、WB及 ROC曲线分析提示其可能是潜在分子标记物；进一步实验显示，SOD3主要在前列腺上皮及间质细胞中表达，但在CP/CPPS前列腺液（EPS）中表达下降；功能研究发现，SOD3是一个Zn离子相关蛋白，并与其它差异蛋白有相互作用。我们推测：血清中高表达的SOD3来自EPS，并通过调控一个蛋白网络参与CP/CPPS发病。本研究拟用质谱为基础的Super SILAC定量蛋白质组学技术，结合改进的免疫共沉淀，研究人EPS中SOD3相互作用蛋白；同时，构建CP/CPPS动物模型，研究SOD3在前列腺上皮及间质细胞中的调控网络，并将获得的结果，通过体内外的实验进行验证及功能研究，最终明确SOD3的蛋白网络调控机制。

Category Ⅲ prostatitis (CP/CPPS) is the most common prostate disease worldwide. Our previous study confirmed SOD3 expression is higher in CP/CPPS patients’ sera than those in the control group by using iTRAQ-2DLC-MALDI-MS/MS. Wb, ELISA and ROC curve analysis showed that SOD3 could be a potential molecular marker. In the further study, SOD3 expresses in both epithelial and stromal cells of prostate. However, the concentration of SOD3 is significantly decreased in EPS of patients with CP/CPPS. Moreover, functional studies suggested that its mechanism is associated with Zinc ions, and it is associated with other differential expressed proteins. Based on above, we speculate that the SOD3 in sera comes from the EPS, and it may involved in the pathogenesis of CP/CPPS with its interaction counterparts. We are going to identify the protein interaction network of SOD3 in human EPS and the epithelial/stromal cells of prostate in the CP/CPPS rats using co-immunoprecipitation and mass spectrometry-based quantitative proteomics with Super SILAC. After that, validation and functional studies will be performed in vivo or in vitro. This work will help to provide a light on the role of SOD3 associated network in CP/CPPS.