渤海典型污染物镉、砷影响菲律宾蛤仔能量代谢的机制研究

镉、砷已成为渤海海洋环境两种典型的重金属污染物，对渤海海洋生态系统及人类健康造成了潜在风险。申请人课题组前期利用蛋白质组学与代谢组学技术，在研究不同重金属对菲律宾蛤仔的毒理效应过程中发现，不同重金属影响的能量代谢途径具有显著的差别，显示蛤仔机体通过不同的策略，来应对不同重金属导致的能量代谢胁迫。本项目针对渤海镉、砷污染现状，拟在前期取得的重金属污染物生态毒理学研究结果基础上，开展镉、砷对菲律宾蛤仔能量代谢影响机制的创新性研究：利用细胞生物学与亚细胞蛋白质组学技术，从菲律宾蛤仔能量代谢细胞器线粒体形态、功能着手，深入阐明渤海典型重金属污染物镉、砷对蛤仔的能量代谢影响的作用机制，挖掘与之相关的蛋白质生物标志物，并用于渤海镉、砷污染的评估。同时，结合前期代谢组学研究结果，构建由镉、砷诱导的能量代谢途径异常的响应指纹图谱，为渤海镉、砷污染的生物监测和风险评估提供有力的工具。

Cadmium (Cd) and arsenic (As) have been typical heavy metal pollutants in the Bohai Sea, which has posed potential risk on the marine ecosystem and human health. Funded by NSFC, our research group previously investigated the toxicological effects of heavy metals in clam Ruditapes philippinarum. Our primary results indicated that heavy metals induced prominent disturbances in energy metabolism via different metabolic pathways in clams. These findings suggested that there existed different strategies in clams to deal with the stress in energy metabolism induced by heavy metals. On the basis of our previous studies in marine ecotoxicology, this project will focus on the pollution of Cd and As in the Bohai Sea to carry out exploratory investigations on the mechanisms of disturbed energy metabolism induced by Cd and As in clam Ruditapes philippinarum. We will integrate cytobiology and subcellular proteomics to characterize the alterations of morphology and functions of the energy metabolism-related organelle, mitochondria, in clams to elucidate the the mechanisms of Cd- and As-induced disturbance in energy metabolism and the proteomic biomarkers that will be then used to assess the field pollution of Cd and As in the Bohai Sea. Combined with the metabolomic biomarkers related Cd and As to clams Ruditapes philippinarum in our previous studies, we will construct the fingerprints of metabolic pathways in energy metabolism disturbed by Cd and As in clams. The work in this project will hopefully provide a powerful tool for the biomonitoring and risk assessment of Cd and As in the Bohai Sea.